Electron Paramagnetic Resonance (EPR)/Q-Band ENDOR Spectrometer

电子顺磁共振 (EPR)/Q 波段 ENDOR 波谱仪

• 批准号: 7125661
• 负责人: Bruce H Robinson
• 金额: $ 104.07万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-05-01 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7125661
• 关键词: Active Sites; Binding Proteins; Biology; Biomedical Research; Catalytic Domain; Cell Nucleus; Crystallography; DNA; Deer; Electron Nuclear Double Resonance; Electron Spin Resonance Spectroscopy; Electrons; Environment; Enzymes; Frequencies; Funding; Gene Expression; Health; Human; Ions; Label; Lipase; Lipids; Location; Magnetic Resonance; Membrane; Numbers; Oregon; Pacific Northwest; Play; Proteins; Protons; RNA; Recovery; Research; Research Personnel; Resolution; Role; Site; Spectrum Analysis; Spin Labels; Standards of Weights and Measures; Structural Protein; Structure; System; Tooth structure; Transition Elements; Wood material; bone; protein structure; research study; three dimensional structure

DESCRIPTION (provided by applicant): Electron paramagnetic resonance (EPR) has long been a standard form of spectroscopy used in biomedical research. EPR has been used to study the function of enzymes, structural proteins and enzymes at interfaces, DMA, and RNA. Over one third of all enzymes, many of which play critical roles in human health, contain transition-metals at the catalytic site. EPR is especially convenient for probing transition metal ion active sites in biology. The information obtained from EPR from the catalytic site is very important to understand the reactivity of the enzyme and its function. Structural proteins and lipids control the health and functionality of membranes. Multi-frequency saturation-recovery (SR) EPR has proven very powerful in studying membranes and membrane-bound proteins. The EPR parameters obtained provide information about dynamics and structure. The relation of an enzyme to its interface (such as lipases, and proteins that control bone and teeth formation) as well as the tertiary structure of RNA, which is integral to gene expression, can be determined using site directed spin labeling. Double electron-electron resonance (DEER or P-ELDOR) experiments allow one to obtain information regarding distances separating two paramagnetic species, such as an NO-label and a transition-metal ion. In this way one can determine the context of the three-dimensional structure of a biomolecule such as DNA, RNA, or a protein, and its relation to its environment. ENDOR (electron nuclear double resonance) is a type of EPR spectroscopy that provides information regarding the nuclei surrounding a paramagnetic center. ENDOR is used to determine protein structure in the absence of a crystal structure, and to define the location of protons (or other nuclei) that might play a critical role in mechanism, yet are difficult to locate by crystallography or EXAFS. Q-Band ENDOR allows nuclei to be located with a high resolution. Replacement of the outdated UW EPR (the only EPR spectrometer in the UW system), combined with an upgrade to include multi-frequency SR, DEER, and Q-band ENDOR capabilities, will facilitate the NIH-funded research of a number of investigators at UW (Robinson, Kovacs, Gelb, Atkins, Stayton, Woods, Fang) as well as neighboring labs (PNNL (Bowman), and U. of Oregon (DeRose)) bringing the pacific northwest into the forefront of magnetic resonance research.

描述（由申请人提供）：电子顺磁共振（EPR）长期以来一直是生物医学研究中使用的标准形式。 EPR已用于研究界面，DMA和RNA的酶，结构蛋白和酶的功能。超过三分之一的酶（其中许多酶在人类健康中都起着关键作用，都包含催化部位的过渡金属。 EPR对于探测生物学中的过渡金属离子活性位点特别方便。从催化位点获得的信息对于了解酶及其功能的反应性非常重要。结构蛋白和脂质控制膜的健康和功能。多频率饱和回收（SR）EPR在研究膜和膜结合的蛋白方面非常有力。获得的EPR参数提供了有关动态和结构的信息。可以使用位点定向自旋标记确定酶与其界面（例如脂肪酶和控制骨和牙齿形成的蛋白）以及RNA的三级结构的关系。双电子电子共振（鹿或p-遗传器）实验允许一个人获得有关将两种顺磁性物种（例如无标记和过渡金属离子）分开的距离的信息。以这种方式，人们可以确定生物分子（例如DNA，RNA或蛋白质）的三维结构的背景及其与环境的关系。 Endor（电子核双共振）是一种EPR光谱，可提供有关围绕顺磁心中心的细胞核的信息。 Endor用于在没有晶体结构的情况下确定蛋白质结构，并定义质子（或其他核）的位置，这些质子（或其他核）可能在机制中起关键作用，但很难通过晶体学或EXAF来定位。 Q波段Endor允许核以高分辨率定位。 Replacement of the outdated UW EPR (the only EPR spectrometer in the UW system), combined with an upgrade to include multi-frequency SR, DEER, and Q-band ENDOR capabilities, will facilitate the NIH-funded research of a number of investigators at UW (Robinson, Kovacs, Gelb, Atkins, Stayton, Woods, Fang) as well as neighboring labs (PNNL （Bowman）和俄勒冈州（Derose））将太平洋西北带入了磁共振研究的最前沿。