HPLC time-of-flight mass spectrometer for accurate mass measurements

用于精确质量测量的 HPLC 飞行时间质谱仪

基本信息

批准号：
7215362
负责人：
RICHARD B VAN BREEMEN
金额：
$ 28.99万
依托单位：
UNIVERSITY OF ILLINOIS AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-05-01 至 2009-04-30
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): A high performance liquid chromatograph time-of-flight (LC-TOF) mass spectrometer will be purchased and used for accurate mass measurements in support of the research of a multidisciplinary group of NIH-funded users (including two multi-investigator center projects). In addition, minor users and new users at the University of Illinois at Chicago as well as nearby Northwestern University and the University of Chicago will be provided access to the proposed LC-TOF mass spectrometer. The LC-TOF mass spectrometer will complement the capabilities of an existing JEOL GCMate mass spectrometer by providing accurate mass measurements using electrospray and/or atmospheric pressure chemical ionization (APCI). Electrospray, APCI, and HPLC are not available on the GCMate. In addition, the new LC-TOF system will provide combined accurate mass measurement with on- line HPLC separation, which is not currently available to this user group, as well as higher throughput than our existing instrumentation. The LC-TOF mass spectrometer will be located within a campus-wide shared instrumentation laboratory called the Research Resources Center (RRC). The RRC is a centralized campus instrumentation facility that has been in operation for over 50 years and is under the oversight of the Vice Chancellor for Research. Access to the instrument by new and continuing users on and off campus will be assured through the administrative oversight of an advisory committee appointed by the RRC. In anticipation of the expansion of our accurate mass measurement service, the RRC recently hired a Ph.D. level mass spectrometrist to oversee the operation of the new LC-TOF mass spectrometer. This new staff position is committed for at least the useful lifetime of the proposed LC-TOF mass spectrometer. In support of this and other Ph.D.-level mass spectrometrists at the RRC, the P.I. (who has more than 20 years of experience managing mass spectrometry facilities) provides oversight to the RRC and advice to the users of the RRC Mass Spectrometry Laboratory. These on-going personnel commitments represent substantial support by the university. User fees (determined by the advisory committee) will be collected to defray the costs of consumable supplies and repairs. Overall, the proposed LC-TOF mass spectrometer will expand our capacity to provide accurate mass measurements to natural product chemists and synthetic organic chemists in the user community who rely upon rapid analyses of their compounds in order to advance with their NIH-funded research programs

描述（由申请人提供）：将购买一台高性能液相色谱飞行时间 (LC-TOF) 质谱仪并用于精确的质量测量，以支持 NIH 资助的用户多学科小组（包括两名多研究者中心项目）。此外，伊利诺伊大学芝加哥分校以及附近的西北大学和芝加哥大学的小用户和新用户将可以使用拟议的 LC-TOF 质谱仪。 LC-TOF 质谱仪将通过使用电喷雾和/或大气压化学电离 (APCI) 提供精确的质量测量来补充现有 JEOL GCMate 质谱仪的功能。 GCMate 上不提供电喷雾、APCI 和 HPLC。此外，新的 LC-TOF 系统将提供精确的质量测量与在线 HPLC 分离的组合（目前该用户组尚无法实现），并且比我们现有的仪器具有更高的通量。 LC-TOF 质谱仪将位于全校园的共享仪器实验室内，称为研究资源中心 (RRC)。 RRC 是一个集中式校园仪器设施，已运行 50 多年，并在研究副校长的监督下。 RRC 任命的咨询委员会的行政监督将确保校园内外的新用户和持续用户能够使用该工具。为了扩大我们的精确质量测量服务，RRC 最近聘请了一位博士。一级质谱师负责监督新型 LC-TOF 质谱仪的运行。这一新的员工职位至少在拟议的 LC-TOF 质谱仪的使用寿命内负责。为了支持这位以及 RRC 的其他博士级质谱师，P.I. （拥有 20 多年管理质谱设施的经验）对 RRC 进行监督，并向 RRC 质谱实验室的用户提供建议。这些持续的人员承诺代表了大学的大力支持。将收取用户费用（由咨询委员会确定）以支付消耗品和维修费用。总体而言，拟议的 LC-TOF 质谱仪将扩大我们的能力，为用户社区中的天然产物化学家和合成有机化学家提供精确的质量测量，这些化学家依靠对其化合物的快速分析来推进 NIH 资助的研究项目