An Accurate Mass TOF Mass Spectrometer - HPLC System for Marine Organic Chemistry

精确质量 TOF 质谱仪 - 用于海洋有机化学的 HPLC 系统

基本信息

批准号：
8246778
负责人：
William Fenical
金额：
$ 32.45万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-05-15 至 2013-05-14
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): To support critical NIH-funded biomedical research at Scripps Institution of Oceanography (SIO) / University of California, San Diego (UCSD), this proposal details need, provides justification and requests funding for a combined high resolution HPLC and high resolution time-of-flight (TOF) mass spectrometer system for rapid, efficient separation of sample components and accurate mass determination of a wide range of molecules derived from this research. The analytical instrumentation includes a high resolution HPLC that can readily accommodate less than 2 micron particle stationary phases operated at pressures up to 9000 psi (600 bar), which provide for effective and efficient several minute analyses of moderately to highly complex mixtures. The requested instrumentation also includes a high resolution (HR) TOF mass spectrometer (MS) with a highly sensitive electrospray ionization source or a combined atmospheric pressure electrospray and chemical ionization source which yields molecular formulas with sub-1 ppm accuracy of unknown and known compounds ranging in polarity from nonpolar organic molecules to highly-charged water-soluble molecules with mass-to-charge ratios of up to 20,000. In a multi-user, "walk- up" environment that is supported by user-friendly open-access software, this LC / HRMS system will be utilized extensively for the rapid determination of molecular formulas for novel marine natural products, efficient "dereplication" of known natural products from marine bacteria and cyanobacteria, identification of microbial metabolites in low concentrations following genetic manipulation, and structure determination of semisynthetic, synthetic and genetically engineered natural products. PUBLIC HEALTH RELEVANCE: This application requests NIH support for the replacement of an aging and obsolete Hewlett-Packard (Agilent) 1100 low resolution LC-MS with an updated Agilent 6230AA LC-MS to be used by NIH sponsored marine biomedical researchers. The proposed instrument, which will be located in the CMBB core, will provide molecular formula data and dramatically shorten analysis times, and accelerate the research of more than 40 faculty, staff, students and postdocs within the Center for Marine Biotechnology and Biomedicine (CMBB) at SIO.

描述（由申请人提供）：为了支持斯克里普斯海洋学研究所 (SIO)/加州大学圣地亚哥分校 (UCSD) 的关键生物医学研究，本提案详细说明了需求，提供了理由并请求为组合高分辨率 HPLC 提供资金高分辨率飞行时间 (TOF) 质谱仪系统，可快速、高效地分离样品成分，并对源自本研究的各种分子进行准确的质量测定。分析仪器包括高分辨率 HPLC，可轻松容纳小于 2 微米的颗粒固定相，在高达 9000 psi (600 bar) 的压力下运行，可对中等到高度复杂的混合物进行有效且高效的几分钟分析。所需的仪器还包括高分辨率 (HR) TOF 质谱仪 (MS)，具有高灵敏度电喷雾电离源或组合大气压电喷雾和化学电离源，可产生未知和已知化合物的分子式，精度低于 1 ppm极性从非极性有机分子到质荷比高达 20,000 的高电荷水溶性分子。在用户友好的开放式软件支持的多用户“直接”环境中，该 LC/HRMS 系统将广泛用于快速确定新型海洋天然产物的分子式、高效的“去重复”海洋细菌和蓝细菌的已知天然产物的鉴定，基因操作后低浓度微生物代谢物的鉴定，以及半合成、合成和基因工程天然产物的结构测定。公共健康相关性：本申请请求 NIH 支持使用更新的 Agilent 6230AA LC-MS 更换老化和过时的 Hewlett-Packard (Agilent) 1100 低分辨率 LC-MS，以供 NIH 资助的海洋生物医学研究人员使用。拟议的仪器将位于 CMBB 核心，将提供分子式数据并大大缩短分析时间，并加速海洋生物技术和生物医学中心 (CMBB) 内 40 多名教职员工、学生和博士后的研究在SIO。