An Accurate Mass TOF Mass Spectrometer - HPLC System for Marine Organic Chemistry

精确质量 TOF 质谱仪 - 用于海洋有机化学的 HPLC 系统

基本信息

批准号：
8246778
负责人：
William Fenical
金额：
$ 32.45万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-05-15 至 2013-05-14
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): To support critical NIH-funded biomedical research at Scripps Institution of Oceanography (SIO) / University of California, San Diego (UCSD), this proposal details need, provides justification and requests funding for a combined high resolution HPLC and high resolution time-of-flight (TOF) mass spectrometer system for rapid, efficient separation of sample components and accurate mass determination of a wide range of molecules derived from this research. The analytical instrumentation includes a high resolution HPLC that can readily accommodate less than 2 micron particle stationary phases operated at pressures up to 9000 psi (600 bar), which provide for effective and efficient several minute analyses of moderately to highly complex mixtures. The requested instrumentation also includes a high resolution (HR) TOF mass spectrometer (MS) with a highly sensitive electrospray ionization source or a combined atmospheric pressure electrospray and chemical ionization source which yields molecular formulas with sub-1 ppm accuracy of unknown and known compounds ranging in polarity from nonpolar organic molecules to highly-charged water-soluble molecules with mass-to-charge ratios of up to 20,000. In a multi-user, "walk- up" environment that is supported by user-friendly open-access software, this LC / HRMS system will be utilized extensively for the rapid determination of molecular formulas for novel marine natural products, efficient "dereplication" of known natural products from marine bacteria and cyanobacteria, identification of microbial metabolites in low concentrations following genetic manipulation, and structure determination of semisynthetic, synthetic and genetically engineered natural products. PUBLIC HEALTH RELEVANCE: This application requests NIH support for the replacement of an aging and obsolete Hewlett-Packard (Agilent) 1100 low resolution LC-MS with an updated Agilent 6230AA LC-MS to be used by NIH sponsored marine biomedical researchers. The proposed instrument, which will be located in the CMBB core, will provide molecular formula data and dramatically shorten analysis times, and accelerate the research of more than 40 faculty, staff, students and postdocs within the Center for Marine Biotechnology and Biomedicine (CMBB) at SIO.

描述（由申请人提供）：为了支持Scripps海洋学机构（SIO） /加利福尼亚大学圣地亚哥分校（UCSD）的关键基金会的重要生物医学研究，此提案细节需要，为高分辨率HPLC和高分辨率的质量分离（TOF）质量分离的组合提供了合理性，并要求提供资金源自这项研究。分析仪器包括一个高分辨率HPLC，可容易地容纳低于2微米的粒子固定相，该固定相的压力最高为9000 psi（600 bar），可提供有效，有效的几分钟分析，对高度复杂的混合物进行了中等程度的分析。所需的仪器还包括具有高度敏感的电喷雾电离源的高分辨率（HR）TOF质谱仪（MS），或者是大气压力电喷雾和化学电离源的组合，该源具有未知数和已知的非层状分子型水的质量和已知化合物的分子式，具有亚1 ppm的精度。最多20,000。在由用户友好的开放式访问软件支持的多用户“漫步”环境中，该LC / HRMS系统将广泛用于快速确定新型海洋天然产物的分子公式半合成，合成和基因工程的天然产物。公共卫生相关性：本申请要求NIH支持更换老化和过时的惠普 - 帕卡德（Agilent）1100低分辨率LC-MS，并提供了更新的Agilent 6230AA LC-MS，将由NIH赞助的海洋生物医学研究人员使用。所提出的仪器将位于CMBB核心中，将提供分子公式数据并大大缩短分析时间，并加速SIO海洋生物技术和生物医学中心（CMBB）中40多名教职员工，教职员工，学生和博士后的研究。