Core--IMAGING

核心--影像

• 批准号: 6948000
• 负责人: David Nicholls
• 金额: $ 5.11万
• 依托单位: BUCK INSTITUTE FOR RESEARCH ON AGING
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6948000
• 关键词: aging; bioimaging /biomedical imaging; biomedical facility; confocal scanning microscopy; histology; immunocytochemistry; immunofluorescence technique

The Imaging Core will provide centralized microanatomical and imaging resources and services for Buck Institute investigators and colleagues at other institutions. The microanatomical resources available include basic histology (tissue fixation and processing, embedding, and morphological stains), immunohistochemistry and immunocytochemistry (immunoperoxidase or alkaline phosphatase procedures with chromagen development), immunofluorescence, and confocal microscopy with Huygen's deconvolution processing and Bitplane Imaris 3D rendering work. The purpose of this proposal is to expand and extend the Core to incorporate a functional imaging facility, enabling investigators to monitor real-time changes in cell function in their models of aging and aging-related disease processes. A wide range of new projects is planned, ranging from those involving the whole nematode to protein interactions within the nucleus. At the center of this expanded capability is a state-of-the-art confocal Zeiss LSM 510 META microscope equipped for functional imaging. The major request in this component is for a full-time Imaging Core research associate, to be fully trained by the Core Director, Dr. David Nicholls, to assist in the planning, execution and processing of functional imaging with this microscope. Additional requests are for service contracts for the microscope and Ti-sapphire pulsed 2-photon laser and a tissue-culture holding facility adjacent to the microscope. In this way this expensive and complex apparatus can be fully utilized by Buck Institute investigators, and also serve as a regional and national resource for research on aging and age-related diseases .

成像核心将为巴克研究所的研究人员和其他机构的同事提供集中的显微解剖和成像资源和服务。可用的显微解剖资源包括基本组织学（组织固定和处理、包埋和形态染色）、免疫组织化学和免疫细胞化学（免疫过氧化物酶或碱性磷酸酶程序与色原显影）、免疫荧光和使用 Huygen 反卷积处理和 Bitplane Imaris 3D 渲染工作的共焦显微镜。该提案的目的是扩展和扩展核心，以纳入功能成像设施，使研究人员能够监测衰老和衰老相关疾病过程模型中细胞功能的实时变化。计划开展一系列新项目，从涉及整个线虫的项目到细胞核内蛋白质相互作用的项目。这一扩展功能的核心是配备功能成像的最先进的共焦蔡司 LSM 510 META 显微镜。该部分的主要要求是全职 成像核心研究员，将接受核心主任 David Nicholls 博士的全面培训，协助使用该显微镜进行功能成像的规划、执行和处理。其他请求包括显微镜和钛蓝宝石脉冲 2 光子激光器以及显微镜附近的组织培养设施的服务合同。通过这种方式，巴克研究所的研究人员可以充分利用这种昂贵而复杂的设备，并且还可以作为老龄化和年龄相关疾病研究的区域和国家资源。