New cationic polymers for DNA delivery with low toxicity

用于 DNA 递送的新型低毒阳离子聚合物

• 批准号: 2888424
• 金额: --
• 依托单位: University of Glasgow
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2888424
• 关键词: New; cationic; polymers; DNA; delivery

The importance of nucleic acid delivery systems for state-of-the-art therapeutics can hardly be overstated. Thus, the development of novel, biocompatible and efficient delivery systems is highly important for improved medical treatments. Traditional synthetic cationic polymers employed for transfection (such as polyethyleneimines) show good transfection ability, but they most often exhibit high toxicity. The goal of this thesis project is the preparation of two types of new biomimetic cationic cyclopolymers with low toxicity. In both cases, a single precursor can be used to readily generate chemically and structurally diverse polymer derivatives. Furthermore, these polymers will be prepared in a topologically and enantiomerically controlled fashion, which allows for further tuning of their properties. We will develop these functional polymers as novel nucleic acid carriers in the form of polyplexes, polyplex micelles and polymersomes. All the new polymers will then be evaluated for cellular compatibility, cellular uptake and gene expression in various models of prostate cancer cells.

核酸递送系统对最先进的治疗剂的重要性几乎不能被夸大。因此，新型，生物相容性和有效的递送系统的发展对于改善医疗治疗非常重要。用于转染的传统合成阳离子聚合物（例如聚乙烯）具有良好的转染能力，但最常见的是毒性高。该论文项目的目的是制备具有低毒性的两种类型的新型仿生阳离子环食物。在这两种情况下，单个前体都可以用来轻松地产生化学和结构上不同的聚合物衍生物。此外，这些聚合物将以拓扑和对映体控制的方式制备，从而进一步调整其性能。我们将以多种形式的多聚胶束，多聚胶束和聚合物体的形式发展这些功能性聚合物作为新型核酸载体。然后，将评估所有新聚合物在各种模型的前列腺癌细胞中的细胞相容性，细胞摄取和基因表达。