SINGLE RNA MOLECULE MOVEMENT VISUALIZED IN LIVING CELLS

活细胞中单 RNA 分子运动的可视化

• 批准号: 7228462
• 负责人: Robert H Singer
• 金额: $ 32.52万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-06-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7228462
• 关键词: Cell Nucleus; Cells; Cytoplasm; Detection; Development; Eukaryotic Cell; Gene Expression; Genetic Transcription; Image; Individual; Life; Mechanics; Metabolism; Methods; Microscopic; Molecular; Movement; Nuclear Export; Process; Proteins; Purpose; RNA; RNA-Protein Interaction; Research Personnel; Scheme; Speed; Technology; Testing; Time; Transcript; Translating; Translations; Work; photoactivation; single molecule; software development

DESCRIPTION (provided by applicant): The purpose of this proposal is to build on our development of single molecule RNA detection in living cells in real time. 1. To extend the technology for fast 4D imaging to describe the movements of RNA transcripts in the nucleus and cytoplasm. 2. To develop a method for detecting translation of single RNA molecules. To image RNA transcription in living cells and follow the individual transcripts released using high speed microscopic 4D imaging to correlate the molecular steps of processing, export and localization with the spatial and temporal colocalization with specific proteins. This work will result in the ability to define the mechanics of RNA metabolism (nuclear export, cytoplasmic transport, localization, translation and degradation) important for the normal functioning of all eukaryotic cells within a spatial and temporal cellular context heretofore not possible using current methods.

描述（由申请人提供）：本提案的目的是建立在我们对活细胞中单分子 RNA 实时检测的开发之上。 1. 扩展快速 4D 成像技术来描述 RNA 转录本在细胞核和细胞质中的运动。 2. 开发检测单个RNA分子翻译的方法。 对活细胞中的 RNA 转录进行成像，并使用高速显微 4D 成像跟踪释放的单个转录本，将处理、输出和定位的分子步骤与特定蛋白质的空间和时间共定位关联起来。这项工作将导致能够定义 RNA 代谢机制（核输出、细胞质运输、定位、翻译和降解），这对于空间和时间细胞环境中所有真核细胞的正常功能非常重要，迄今为止使用现有方法是不可能的。