Reducing antimicrobial resistant bacteria with the use of cold atmospheric plasma: Mechanism of action and influence on mutation and horizontal gene t

使用冷大气等离子体减少抗菌药物耐药性细菌：作用机制以及对突变和水平基因t的影响

• 批准号: 2888329
• 金额: --
• 依托单位: University of Bristol
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2888329
• 关键词: Reducing; antimicrobial; resistant; bacteria; use

Project description:The widespread use and misuse of antibiotics in animal production has led to the emergence of antimicrobial-resistantAMR bacteria on farms, which adds to the global burden of AMR, which has significant impacts on human health andfood security. Significant efforts to limit AMR on animal farms have so far focused on controlling the supply and use ofantimicrobial drugs. However, evidence shows that AMR pathogenic bacteria can persist and spread despite reducingantimicrobial use to almost zero. AMR development and persistence in livestock is therefore not only an issue to beaddressed by reducing antimicrobial use. We have shown that cleaning and disinfection have a very important role toplay, but the extensive use of chemical disinfectants has been associated with the emergence of AMR and has aconsiderable environmental impact. Therefore, new sustainable strategies for decontamination at the farm level arenecessary. Cold atmospheric plasma (CAP) is a novel, residue and antibiotic-free, non-thermal technology shown to haveantibacterial properties in a variety of different settings. Plasma describes the state of an ionised gas (4th state ofmatter), with natural examples including the northern lights, lightening and solar winds. In our lab, we produce CAP byexcitation of gas molecules using electrical discharges. CAP has the potential to inhibit bacteria due to the reactivemolecules and species it contains (e.g. oxygen/nitrogen reactive species). Our previous studies have proved that CAP iseffective in eliminating bacterial pathogens on different surfaces. This project aims to explore the viability of using CAPto reduce AMR bacterial contamination on surfaces frequently found on farms, and the biological effects of CAP onbacteria. You will investigate: i) the efficacy of CAP against AMR bacteria previously identified on farms; ii) the CAP-mediated mechanism of action against AMR bacteria using functional genomics; iii) The influence of CAP on mutationand horizontal transfer of AMR in bacteria; iv) the efficacy of CAP in controlling AMR bacteria on abiotic surfaces. The successful student will join a consortium of two interdisciplinary research groups with access to world-class researchfacilities at University of Bristol (molecular bacteriology, proteomics/functional genomics) and University of the West ofEngland-Bristol (cold plasma technology, microbiology). The student will receive excellent training and meaningfulsupport from their supervisory team, and develop the technical skills and enterprising mind-set that employers seek.

项目描述：动物生产中抗生素的广泛使用和滥用导致在农场上出现了抗菌抗药性细菌，这增加了AMR的全球负担，这对人类健康和食物的安全产生了重大影响。迄今为止，限制AMR限制AMR的巨大努力集中在控制抗微生物药物的供应和使用上。然而，有证据表明，尽管减少了抗药性的用途，AMR致病细菌仍可以持续并扩散。因此，牲畜中的AMR发展和持久性不仅是减少抗菌使用的问题。我们已经表明，清洁和消毒具有非常重要的作用，但是对化学消毒剂的广泛使用与AMR的出现有关，并且具有可观的环境影响。因此，需要新的可持续策略在农场层面上进行净化。冷大气血浆（CAP）是一种新型，残留和无抗生素的非热技术，在各种不同的环境中都显示出易于Haveantibacterial特性。血浆描述了一个离子气体的状态（第4个状态），其中包括北极光，闪电和太阳风在内的自然例子。在我们的实验室中，我们使用电放电对气体分子产生帽限制。 CAP有可能抑制由于其包含的重新分子及其物种引起的细菌（例如氧/氮反应性物种）。我们以前的研究证明，帽可在消除不同表面上的细菌病原体方面有效。该项目旨在探索使用CARTO减少在农场上经常发现的表面上的AMR细菌污染以及帽细菌的生物学作用的生存能力。您将调查：i）CAP对以前在农场上鉴定出的AMR细菌的功效； ii）使用功能基因组学对AMR细菌的帽介导的作用机理； iii）CAP对细菌中AMR突变和水平转移的影响； iv）CAP在非生物表面控制AMR细菌方面的功效。成功的学生将加入两个跨学科研究小组的联盟，并在布里斯托尔大学（分子细菌学，蛋白质组学/功能基因组学）和恩格兰 - 布里斯托尔大学（Cold Plasma-Bristol-Bristol Technology of Bristol of Bristol of Bristol of Bristol of Bristol of Bristol of Bristoligationsfiessions）。该学生将从其监督团队中获得出色的培训和有意义的支持，并发展雇主寻求的技术技能和进取心。