EPC mediated Neovascularization in Ischemic Myocardium

EPC介导的缺血心肌新生血管形成

• 批准号: 6885190
• 负责人: PAVAN ATLURI
• 金额: $ 4.99万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-02-01 至 2006-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6885190
• 关键词: CD34 molecule; angiogenesis; antibody; cell differentiation; cell migration; cell proliferation; chemokine; colony stimulating factor; flow cytometry; gene therapy; heart catheterization; histopathology; immunocytochemistry; laboratory rat; microcapsule; muscle contraction; myocardial infarction; myocardial ischemia /hypoxia; perfusion; postdoctoral investigator; proliferating cell nuclear antigen; radiosensitizer; stem cells; von Willebrand factor

DESCRIPTION (provided by applicant): Ischemic cardiomyopathy (ICM) is an increasingly prevalent global health concern. Endothelial progenitor cell (EPC) mediated neo-vascularization may provide a means to increase collateral circulation in ischemic myocardium, thereby improving cardiac function. A strategy of global bone marrow stimulation with granulocyte-monocyte colony stimulating factor (GM-CSF) to induce EPC proliferation combined with stromal cell-derived factor (SDF) targeted myocardial EPC chemokinesis will be utilized to enhance neovasculogenesis within ischemic myocardium. Initial data obtained utilizing an established rat model of ICM has demonstrated enhanced myocardial contractility and neovascularization among rats treated with a combination of GM-CSF and SDF. The proposed research will attempt to elucidate the role of SDF as a novel means of enhancing myocardial neovascularization specifically focusing on EPC mobilization, myocardial perfusion, ischemia reversal, enhanced myocardial function, and limited ventricular remodeling with preservation of geometry.

描述（由申请人提供）：缺血性心肌病（ICM）是一个日益普遍的全球健康问题。内皮祖细胞（EPC）介导的新血管形成可能提供增加缺血心肌侧支循环的方法，从而改善心脏功能。使用粒细胞-单核细胞集落刺激因子 (GM-CSF) 诱导 EPC 增殖的整体骨髓刺激策略与靶向心肌 EPC 趋化作用的基质细胞衍生因子 (SDF) 相结合，将用于增强缺血心肌内的新血管生成。利用已建立的 ICM 大鼠模型获得的初步数据表明，接受 GM-CSF 和 SDF 联合治疗的大鼠的心肌收缩力和新生血管形成增强。拟议的研究将试图阐明 SDF 作为增强心肌新生血管形成的新方法的作用，特别关注 EPC 动员、心肌灌注、缺血逆转、增强心肌功能和保留几何形状的有限心室重塑。