Luminescence Imaging: Molecules, Cells and Tissues

发光成像：分子、细胞和组织

基本信息

批准号：
7047193
负责人：
David C Spray
金额：
$ 20.88万
依托单位：
ALBERT EINSTEIN COLLEGE OF MEDICINE
依托单位国家：
美国
项目类别：
财政年份：
2006
资助国家：
美国
起止时间：
2006-05-15 至 2009-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7047193
关键词：
astrocytes astrocytoma bone marrow cytokine electromagnetic radiation gap junctions gene expression genes housing light intensity luminescence medicine membrane channels tissue /cell culture tissues training transplantation university

项目摘要

DESCRIPTION (provided by applicant): The purpose of this application is to obtain a photon-counting camera and accessories to allow high temporal and spatial resolution of luminescence within cultured cells and in whole animals; accessories include both multiwell plate and single tube luminometers required for optimization of expression and activation of the photoproteins in vitro and an imaging station in which whole animal imaging will be performed. There is no such high sensitivity camera or whole animal imager currently at Einstein College of Medicine, and there is currently neither type of luminometer in the nine-floor Kennedy Center housing the Department of Neuroscience, where the proposed studies will be performed. The studies that are proposed are all natural extensions of NIH-funded projects of nine investigators that will use the relatively new technique of spatially resolved luminescence recording that has become more accessible due to new generations of vector constructs in which the photoproteins have been optimized with regard to intensity and wavelengths of light output and destabilized with regard to gene expression reporting. The studies of users outlined in this proposal are diverse, including bioluminescent resonance energy transfer to determine dimerization and binding partners for connexin molecules, luminescent reporter molecules to localize ATP release from within astrocytes and astrocytoma cells, luminescence-tagged bone marrow cells in order to characterize their tissue distribution following transplantation into mice, and gene promoters fused to luminescent reporter molecules to identify time course of gene activation in cytokine-stimulated astrocytes and to develop a moderately high throughput assay by which to identify connexin-specific gap junction channel blockers. The proposal also includes the plan for a bimonthly user group meeting, through which new users will be attracted to the instrument and new analytical tools and protocols will be discussed, as well as problems that may arise. It also includes plans for training as well as inclusion of relevant methods in a graduate student course.

描述（由申请人提供）：本申请的目的是获得光子计数摄像头和配件，以允许在培养的细胞内以及整个动物中的发光时间高和空间分辨率；配件包括在体外优化表达和激活所需的多井板和单管发光仪，以及将在其中进行整个动物成像的成像站。爱因斯坦医学院目前没有如此高的灵敏度摄像头或全动物成像仪，目前在九楼的肯尼迪中心住房中既没有类型的发光仪计算机计，则将进行拟议的研究。提出的研究都是NIH资助的九个研究者项目的全部自然扩展，这些项目将使用相对较新的空间分辨的发光记录技术，由于新一代的向量构建体变得更加易于访问，在这些媒介构建体中，光蛋白已针对光的强度和典型的发光和巨大的势头表达报告而优化了光蛋白，并且对基因表达的表达相关。 The studies of users outlined in this proposal are diverse, including bioluminescent resonance energy transfer to determine dimerization and binding partners for connexin molecules, luminescent reporter molecules to localize ATP release from within astrocytes and astrocytoma cells, luminescence-tagged bone marrow cells in order to characterize their tissue distribution following transplantation into mice, and gene promoters fused to luminescent记者分子以鉴定细胞因子刺激的星形胶质细胞中基因激活的时间过程，并开发中等高的吞吐量测定法，以鉴定连接蛋白特异性的间隙连接通道阻滞剂。该提案还包括每两个月用户组会议的计划，将通过该计划将新用户吸引到仪器中，并将讨论新的分析工具和协议，以及可能出现的问题。它还包括培训计划以及在研究生课程中包含相关方法。