Urease Activity and Dental Caries in Children

脲酶活性与儿童龋齿

• 批准号: 6871943
• 负责人: EVANGELIA MOROU-BERMUDEZ
• 金额: $ 10.76万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6871943
• 关键词: Puerto Rican; acidity /alkalinity; age difference; bacterial proteins; clinical research; dental caries; dental caries inhibitor; dental deposit removal; dental disorder diagnosis; dental plaque; dietary carbohydrates; disease /disorder proneness /risk; enzyme activity; gender difference; human subject; longitudinal human study; microorganism population study; nucleic acid hybridization; oral bacteria; patient oriented research; preschool child (1-5); saliva; single cell analysis; tissue resource /registry; urease

DESCRIPTION: The Candidate in this revised application for a K23 Award has graduate clinical training in Pediatric Dentistry and PhD level research training in Microbiology and in Cariology. The purpose of the requested Award is to provide the candidate with multidisciplinary training in patient-oriented research, which will help her design and conduct translational studies related to biological predictors of caries risk in children. The Career Development Plan of this proposal consists of a multidisciplinary program, which includes training in the responsible conduct of research, epidemiology, biostatistics and clinical research design, among others. The plan also includes a number of activities that will provide additional hands-on experience in clinical research and continuous training in molecular biology throughout the Award period. The Research component of this application consists of a combined clinical and basic research study, which aims to address the role bacterial urease activity in the development of dental caries in children. Bacterial urease activity in dental plaque generates ammonia, which is believed to inhibit the development of dental caries, and which can also influence the oral microbial ecology. If these relationships can be demonstrated, future studies could lead to the development of novel methods for caries risk assessment and prevention. The primary mentor is Dr. Augusto Elias, Professor and Director of the Research Office in the UPR School of Dentistry. Dr. Elias has extensive experience as a mentor, as a reference examiner in caries studies worldwide, and as the principal investigator of numerous corporate-sponsored dental clinical trials and prevalence studies in Puerto Rico. Dr. Elias will be joined in his role as a mentor by two co-mentors, Dr. Ronald Billings (University of Rochester) and Dr. Robert Burne (University of Florida) who will provide expert advice and technical support to the clinical and the basic science portion of the study, respectively. Dr. Erick Suarez, Professor in Biostatistics, will guide the candidate in study design methodology and in the analysis of the data.

描述：修订后的 K23 奖申请中的候选人接受过儿科牙科研究生临床培训以及微生物学和龋齿学博士级研究培训。所申请奖项的目的是为候选人提供以患者为导向的研究方面的多学科培训，这将有助于她设计和开展与儿童龋齿风险的生物预测因子相关的转化研究。 该提案的职业发展计划由一个多学科计划组成，其中包括负责任的研究行为、流行病学、生物统计学和临床​​研究设计等方面的培训。该计划还包括一系列活动，这些活动将在整个奖励期间提供额外的临床研究实践经验和分子生物学持续培训。 该申请的研究部分包括临床和基础研究相结合，旨在解决细菌脲酶活性在儿童龋齿发展中的作用。牙菌斑中的细菌脲酶活性会产生氨，这被认为可以抑制龋齿的发展，并且还可以影响口腔微生物生态。如果这些关系能够得到证实，未来的研究可能会导致龋齿风险评估和预防的新方法的开发。 主要导师是UPR牙科学院教授兼研究室主任Augusto Elias博士。 Elias 博士作为导师、全球龋齿研究的参考检查员以及波多黎各众多企业赞助的牙科临床试验和患病率研究的首席研究员拥有丰富的经验。 Elias 博士将由两位共同导师 Ronald Billings 博士（罗切斯特大学）和 Robert Burne 博士（佛罗里达大学）共同担任导师，他们将为临床和临床研究提供专家建议和技术支持。分别是该研究的基础科学部分。生物统计学教授 Erick Suarez 博士将指导候选人研究设计方法和数据分析。