Genotypes of Helicobacter Pylori in West Africa

西非幽门螺杆菌的基因型

• 批准号: 6933857
• 负责人: RICHARD A ADEGBOLA
• 金额: $ 5.4万
• 依托单位: MEDICAL RESEARCH COUNCIL UNIT THE GAMBIA AT THE LONDON SCHOOL OF HYGIENE & TROPICAL MEDICINE
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6933857
• 关键词: Africa; Helicobacter; acidity /alkalinity; adult human (21+); age difference; bacterial genetics; biochemical evolution; children; chronic disease /disorder; clinical research; communicable disease transmission; disease /disorder onset; disease /disorder proneness /risk; drug resistance; gastroscopy; gene environment interaction; genetic polymorphism; genetic strain; genotype; host organism interaction; human subject; metronidazole; microorganism classification; microorganism culture; microorganism population study; tube feeding

DESCRIPTION (provided by applicant): Helicobacter pylori infects over half of adults worldwide. Colonization begins in childhood, and contributes to gastroduodenal disease in adult life. Strains obtained close to colonization are rarely encountered, as they require isolation from young children, yet a detailed study of such strains is critical to understanding H. pylori associated disease. Our study will be conducted in The Gambia, W. Africa, the ancestral home of many African Americans, in a population with a high early childhood incidence of H. pylori colonization. The sadly frequent need for the use of naso-gastric tubes to intensively re-feed malnourished infants, and the high early childhood incidence of H. pylori infection, allow us a unique opportunity to investigate colonizing strains of H. pylori, and further develop appropriate local approaches to disease management. Our goal is to evaluate new methods for culturing H. pylori strains from young children, and to compare these to those obtained from adults. We propose that the transition from childhood colonization to adult disease state is affected by multiple factors including evolution of genotypes of colonizing H. pylori strains themselves, so that H. pylori strains from Gambian children will differ genetically from strains from chronically colonized adults. We have two specific aims: Aim 1: To compare the range of genotypes obtained from H. pylori cultured from gastric biopsies from Gambians, with those obtained by culture of gastric juice aspirate, from the same subjects: Aim 2: To assess the relatedness of child strains and those from close adult relatives, cultured from naso-gastric aspirates. The data from these experiments will give us our first insight into the changes that may occur in the H. pylori genome during the period of colonization of its unique niche, the human stomach. We anticipate that our work will allow further understanding of why the clinical diseases associated with chronic H. pylori colonization in West Africa differ from those seen in industrialized nations, and enable the development of appropriate treatment strategies for communities such as The Gambia.

描述（由申请人提供）：幽门螺杆菌感染全世界一半以上的成年人。定植始于儿童时期，并导致成年后的胃十二指肠疾病。接近定植时获得的菌株很少遇到，因为它们需要与幼儿隔离，但对此类菌株的详细研究对于了解幽门螺杆菌相关疾病至关重要。我们的研究将在西非的冈比亚进行，这里是许多非裔美国人的祖籍地，该地区的儿童早期幽门螺旋杆菌定植率很高。不幸的是，频繁需要使用鼻胃管来集中喂养营养不良的婴儿，以及儿童早期幽门螺杆菌感染的高发病率，为我们提供了一个独特的机会来研究幽门螺杆菌的定植菌株，并进一步开发适当的方法。当地的疾病管理方法。 我们的目标是评估从幼儿中培养幽门螺杆菌菌株的新方法，并将这些方法与从成人中获得的方法进行比较。我们认为，从儿童定植到成人疾病状态的转变受到多种因素的影响，包括定植幽门螺杆菌菌株本身基因型的进化，因此来自冈比亚儿童的幽门螺杆菌菌株在遗传上与来自长期定植的成人的菌株不同。我们有两个具体目标： 目标 1：比较冈比亚人胃活检培养的幽门螺杆菌基因型范围与同一受试者通过胃液抽吸物培养获得的基因型范围： 目标 2：评估儿童菌株和来自成年近亲的菌株，通过鼻胃抽吸物培养。这些实验的数据将使我们首次了解幽门螺杆菌基因组在其独特的生态位（人类胃）定植期间可能发生的变化。我们预计我们的工作将有助于进一步了解为什么西非与慢性幽门螺杆菌定植相关的临床疾病与工业化国家的疾病不同，并有助于为冈比亚等社区制定适当的治疗策略。

项目成果

Antimicrobial susceptibility and resistance patterns among Helicobacter pylori strains from The Gambia, West Africa.

西非冈比亚幽门螺杆菌菌株的抗菌敏感性和耐药性模式。

• 发表时间: 2013-03
• 影响因子: 4.9
• 作者: Secka, Ousman;Berg, Douglas E;Antonio, Martin;Corrah, Tumani;Tapgun, Mary;Walton, Robert;Thomas, Vivat;Galano, Juan J;Sancho, Javier;Adegbola, Richard A;Thomas, Julian E
• 通讯作者: Thomas, Julian E

Mixed infection with cagA positive and cagA negative strains of Helicobacter pylori lowers disease burden in The Gambia.

幽门螺杆菌 cagA 阳性和 cagA 阴性菌株的混合感染降低了冈比亚的疾病负担。

• 发表时间: 2011
• 影响因子: 3.7
• 作者: Secka, Ousman;Antonio, Martin;Berg, Douglas E;Tapgun, Mary;Bottomley, Christian;Thomas, Vivat;Walton, Robert;Corrah, Tumani;Thomas, Julian E;Adegbola, Richard A
• 通讯作者: Adegbola, Richard A

Population genetic analyses of Helicobacter pylori isolates from Gambian adults and children.

冈比亚成人和儿童幽门螺杆菌分离株的群体遗传学分析。

• 发表时间: 2014
• 影响因子: 3.7
• 作者: Secka, Ousman;Moodley, Yoshan;Antonio, Martin;Berg, Douglas E;Tapgun, Mary;Walton, Robert;Worwui, Archibald;Thomas, Vivat;Corrah, Tumani;Thomas, Julian E;Adegbola, Richard A
• 通讯作者: Adegbola, Richard A