CHAR OF GLYCANS FROM MOUSE & BOVINE UROPLAKINS IA & IB BY MASS SPECTROMETRY

来自小鼠的聚糖的字符

• 批准号: 7182184
• 负责人: Tung-Tien Sun
• 金额: $ 4.04万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7182184
• 关键词: biomedical equipment resource; biomedical resource; mass spectrometry; method development

DESCRIPTION: MALDI mass spectrometry has become one of the two important methods driving the application of mass spectrometry in biochemistry. A problem that stands in the way of broad application is the extensive metastable-ion decay exhibited by some molecules. This is a particular problem when a class of molecules has one or more labile groups that undergo reactions with significantly lower fragmentation barriers than those for analytically useful fragmentations. An example is the loss of water, for which there is little information content. The problem is made worse when a mixture is encountered. The composite fragmentation is difficult, if not impossible, to assign. A promising development to overcome this limitation is to initiate the desorption process in the presence of a bath gas. This idea will be pursued for the analysis of samples containing oligosaccharides, glyco and phosphopeptides, sialylated glycolipids, sulfated oligosaccharides, and peptides containing oxidized cysteine residues. Many of these samples are of interest to collaborators of the BU research resource.

描述：MALDI质谱已成为推动质谱在生物化学中应用的两种重要方法之一。 妨碍广泛应用的一个问题是一些分子表现出的广泛的亚稳态衰变。 当一类分子具有一个或多个不稳定的组，其反应的碎片障碍明显低于分析有用的碎片，这是一个特殊的问题。 一个例子是缺水，信息内容很少。 当遇到混合物时，问题会变得更糟。 复合碎片很难（即使不是不可能）很难分配。 克服这一限制的有希望的发展是在存在浴气的情况下启动解吸过程。 该想法将被追求，以分析含有寡糖，糖和磷酸肽的样品，溶解的糖脂，硫酸化的寡糖以及含有氧化半胱氨酸残基的肽。 BU研究资源的合作者感兴趣的许多样本都很感兴趣。