Biacore T100

比亚科T100

• 批准号: 7046452
• 负责人: KENDALL L. KNIGHT
• 金额: $ 32万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-30 至 2006-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7046452
• 关键词: biomedical equipment purchase; surface plasmon resonance

DESCRIPTION (provided by applicant): The Biacore T100 is a state-of-the-art surface plasmon resonance instrument that combines high sensitivity with automated high throughput, making it an ideal instrument for the study of biomolecular interactions. No comparable instrument currently exists on campus. The group of investigators is composed of 5 major users, all of whom are NIH funded. All of the users are performing quantitative studies of biomolecular interactions, investigating a broad range of systems, including protein-protein, protein-small molecule.protein-nucleic acid and protein-carbohydrate interactions. Knowledge of the thermodynamic and kinetic parameters of molecular interactions is a critical step in characterizing the regulation and catalysis of numerous biological pathways. The Biacore T100 enables a broad spectrum of experiments including: thermodynamics, solution-affinity, concentration analysis, and kinetics. This instrument is the successor of the Biacore 3000 and allows for rapid screening of multiple ligands to multiple interacting partners under a variety of conditions (concentration, pH, ionic strength, temperature, etc.), and the high sensitivity requires very little sample in cases when amounts of the biomolecules are limiting. This instrument will complement UMass Medical School's institutional commitment for investigating biological macromolecules, which already includes mass spectrometry, microscopy, x-ray crystallography, calorimetry and NMR. The proposed instrument purchase will fill acute research needs for NIH-funded investigators at UMass Medical School.

描述（由申请人提供）：Biacore T100 是一款最先进的表面等离子共振仪器，将高灵敏度与自动化高通量相结合，使其成为研究生物分子相互作用的理想仪器。目前校园内没有类似的仪器。研究人员小组由 5 位主要用户组成，全部由 NIH 资助。所有用户都在进行生物分子相互作用的定量研究，研究广泛的系统，包括蛋白质-蛋白质、蛋白质-小分子、蛋白质-核酸和蛋白质-碳水化合物相互作用。了解分子相互作用的热力学和动力学参数是表征众多生物途径的调节和催化的关键步骤。 Biacore T100 可进行广泛的实验，包括：热力学、溶液亲和力、浓度分析和动力学。该仪器是 Biacore 3000 的后继产品，允许在各种条件（浓度、pH、离子强度、温度等）下快速筛选多个配体到多个相互作用的伴侣，并且在高灵敏度的情况下需要很少的样品当生物分子的数量受到限制时。该仪器将补充麻省大学医学院研究生物大分子的机构承诺，其中包括质谱、显微镜、X 射线晶体学、量热法和核磁共振。拟议购买的仪器将满足美国国立卫生研究院 (NIH) 资助的麻省大学医学院研究人员的迫切研究需求。