Embryonic Stem Cell Research Career Enhancement

胚胎干细胞研究职业提升

基本信息

批准号：
7289100
负责人：
Kenneth S. Zuckerman
金额：
$ 0.65万
依托单位：
H. LEE MOFFITT CANCER CTR & RES INST
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-09-01 至 2007-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7289100
关键词：
Embryonic Stem Cell Research Career

项目摘要

DESCRIPTION: See instructions. State the application's broad, long-term objectives and specific aims, making referenceto the health relatedness of the project (i.e., relevance to the mission of the agency). Describe concisely the research design and methods for achieving these goals. Describe the rationale and techniques you will use to pursue these goals. In addition, in two or three sentences, describe in plain, lay language the relevance of this research to public health. If the application is funded, this description, as is, will become public information. Therefore, do not include proprietary/confidential information. DO NOT EXCEED THE SPACE PROVIDED. This is an application for a supplement to a K18 grant. The purpose of the supplement is to provide funds to permit initiation of embryonic stem cell research in my laboratory to replace the reagents generated during my K18 grant-supported research Waningin Australia, which have become unavailable for transfer to my aboratory due to unforeseen circumstances beyond the control of my research mentor (Dr. Martin Pera), my collaborator in the Pera lab (Dr. Ernst Wolvetang), or me. With this supplemental support, my lab will be able to purchase 3 human US federally approved human embryonic stem cell (hESC) lines, lentiviral expression vectors to transduce the embryonic stem cells, and oligonucleotides (plus sequencing confirmation) for construction of expression vectors. These funds will allow us to extablish growth of hESC lines and to transduce these hESC lines with lentiviral expression vectors. Once we have completed these steps, we will have reconstructed what we created in Australia and will be prepared to initiate our own research program on regulation of hESC sternness and differentiation. PERFORMANCE SITE(S) (organization, city, state) H. Lee Moffitt Cancer Center and Research Institute University of South Florida Tampa, FL PHS 398 (Rev. 04/06) Page 2 Form Page 2 Principal Investigator/Program Director (Last, First, Middle): ZllCkerman, Kenneth S. KEY PERSONNEL. See instructions. Use continuation pages as neededto provide the required information in the formatshown below. Start with Principal Investigator(s).List all other key personnel in alphabetical order, last name first. Name eRA Commons User Name Organization Role on Project Kenneth S. Zuckerman kenzuckerman H. Lee Moffitt Cancer CtrP.I. OTHER SIGNIFICANT CONTRIBUTORS Name Organization Role on Project Human Embryonic Stem Cells Q No ^ Yes If the proposed project involves human embryonic stem cells, list below the registration number of the specific cell line(s) from the following list: http://StemcellS.nih.qov/registrv/index.asp. Usecontinuation pages as needed. If a specific line cannot be referenced at this time,include a statementthat one from the Registry will be used. Cell Line WA01 WA07 WA09 PHS 398 (Rev. 04/06) Page 3 Form Page 2-continued Number the following pages consecutively throughout the application. Do not use suffixes such as 4a, 4b. Principal Investigator/Program Director (Last, First, Middle): ZllCkerman, Kenneth S. The name of the principal investigator/program director must be provided at the top of each printed page and each continuation page. RESEARCH GRANT TABLE OFCONTENTS Page Numbers Face Page 1 Description, Performance Sites, Key Personnel, Other Significant Contributors, and Human Embryonic Stem Cells 2-3

描述：请参阅说明。陈述该应用程序的广泛，长期目标和具体目标，使参考该项目（即与代理机构的任务相关）。简单地描述实现这些目标的研究设计和方法。描述您将用来追求这些目标的基本原理和技术。此外，用两个或三个句子描述，以简单的语言描述这项研究与公共卫生的相关性。如果申请是资助的，则描述将成为公共信息。因此，请勿包含专有/机密信息。不要超过空间假如。这是对K18赠款补充的申请。补充的目的是向允许在我的实验室中启动胚胎干细胞研究，以取代我的K18赠款支持的研究Waningin澳大利亚，这已经无法转移到我的由于无法预料的情况，我的研究导师（Martin Pera博士），我 Pera Lab（Ernst Wolvetang博士）或我的合作者。通过这种补充支持，我的实验室将能够购买3种人类联邦批准的人类胚胎干细胞（HESC）线，慢病毒表达传递胚胎干细胞和寡核苷酸的载体（加上测序确认）表达向量的构造。这些资金将使我们能够扩展hESC线的增长和用慢病毒表达向量转导这些hESC线。完成这些步骤后，我们将已经重建了我们在澳大利亚创造的内容，并准备启动自己的研究计划关于hESC的严厉和分化的调节。绩效网站（组织，城市，州） H. Lee Moffitt癌症中心和研究所南佛罗里达大学坦帕，佛罗里达州 PHS 398（修订版04/06）第2页表格页2 首席研究员/计划主任（最后，第一，中间）：Zllckerman，Kenneth S. 关键人员。请参阅说明。根据需要使用延续页面，以下面的格式提供所需的信息。从首席调查员开始。按字母顺序排列所有其他关键人员，首先要姓氏。名称ERA CONSONS用户名组织在项目中的角色 Kenneth S. Zuckerman Kenzuckerman H. Lee Moffitt Cancer Ctrp.I. 其他重要的贡献者姓名组织角色人类胚胎干细胞q否 ^是如果所提出的项目涉及人类胚胎干细胞，请从以下列表中列出特定细胞系的注册数： http：//stemcells.nih.qov/registrv/index.asp。根据需要进行用户关注页。如果目前无法引用特定行，则将使用注册表中的一条语句。细胞系 WA01 WA07 WA09 PHS 398（修订版04/06）第3页表格第2页。在整个过程中连续编号以下页面应用程序。请勿使用4A，4B等后缀。首席研究员/计划主任（最后，第一，中间）：Zllckerman，Kenneth S. 必须在每个印刷页面的顶部和每个延续页面的顶部提供主要调查员/计划主管的名称。研究赠款目录页码面部第1页描述，绩效站点，关键人员，其他重要贡献者和人类胚胎干细胞2-3