Fiber Optic Array Based Bioluminesence Imaging

基于光纤阵列的生物发光成像

• 批准号: 6990784
• 负责人: MICHAEL Spencer KOLODNEY
• 金额: $ 10万
• 依托单位: KORIMA, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-23 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6990784
• 关键词: SCID mouse; bioimaging /biomedical imaging; bioluminescence; biomedical equipment development; charge coupled device camera; computer program /software; computer system design /evaluation; fiber optics

DESCRIPTION (provided by applicant): Bioluminescence imaging is a novel technique for visualizing biological processes within living animals. The cells or organism of interest are genetically modified to express luciferase, the enzyme that causes some insects, jellyfish, and bacteria to glow. Luciferase catalyzes a chemical reaction that generates light as one of its products. A highly sensitive camera captures this light and produces a semi-quantitative image reflecting cell trafficking, proliferation or gene expression in the living animal. Thus, by measuring light emission, one can monitor cellular or genetic activity and use the results to monitor the spread of a disease, or the effect of a new drug candidate in vivo. Only two companies (Xenogen, Alameda, CA and Photometries, Tucson AZ) currently manufacture equipment designed for bioluminescence imaging. Their approach uses a low f-number lens to collect about 0.2% of the photons emitted from the animal. We propose using a tapered fiber optic image conduit in place of a lens to increase photon collection efficiency. We hypothesize that this approach should increase the sensitivity of bioluminescence by capturing nearly all of the emitted photons. Improvements in the ability to detect signals in real time in live animals will ultimately result in better data, at lower cost, using fewer animals.

描述（由申请人提供）：生物发光成像是一种用于可视化活体动物内生物过程的新技术。感兴趣的细胞或生物体经过基因改造以表达荧光素酶，这种酶可使一些昆虫、水母和细菌发光。荧光素酶催化化学反应，产生光作为其产物之一。高灵敏度相机捕获这种光并产生反映活体动物中细胞运输、增殖或基因表达的半定量图像。因此，通过测量光发射，人们可以监测细胞或遗传活动，并使用结果来监测疾病的传播或新候选药物在体内的作用。目前只有两家公司（加利福尼亚州阿拉米达的 Xenogen 和亚利桑那州图森的 Photometries）制造用于生物发光成像的设备。他们的方法使用低光圈值透镜来收集动物发出的约 0.2% 的光子。我们建议使用锥形光纤图像导管代替透镜以提高光子收集效率。我们假设这种方法应该通过捕获几乎所有发射的光子来提高生物发光的灵敏度。实时检测活体动物信号的能力的提高最终将导致使用更少的动物、以更低的成本获得更好的数据。