Confocal Microscope for Core Imaging Facility

用于岩心成像设施的共焦显微镜

• 批准号: 6877361
• 负责人: Chi-Bin Chien
• 金额: $ 44.67万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877361
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; confocal scanning microscopy; molecular /cellular imaging

DESCRIPTION (provided by applicant): Imaging of biological specimens has become an increasingly important tool in biomedical science, with the abilities to image live specimens and multiple fluorophores being especially important. We propose to purchase a Zeiss LSM510 confocal microscope system to be housed in the Cell Imaging Facility of the University of Utah School of Medicine. This confocal would replace an obsolete entry-level confocal (Olympus FVX) and improve the capabilities of the facility by adding a wide choice of excitation wavelengths, as well as fast switching between multiple emission filters. An environmentally controlled specimen chamber and motorized X-Y stage would particularly facilitate live-cell imaging experiments. No instrument with this set of features is currently available anywhere at the University of Utah. The core Cell Imaging Facility is centrally located in the School of Medicine, and is overseen by a fulltime Ph.D. director with postdoctoral experience. Under his direction the facility has been highly successful, with heavy use of the existing confocal microscopes (>35 hours/week/microscope). The director provides training and support to users in addition to maintaining the microscopes and administering their use. Furthermore, every year he teaches a didactic microscopy course together with the Principal Investigator. There is a strong institutional commitment to providing core microscopy facilities. The University of Utah fully funds the facility director's salary, and guarantees the cost of service contracts. The instrument's use would be monitored by an oversight committee, which would liaise with a committee that oversees all of the School of Medicine's core facilities. This instrument would primarily serve 7 major users from 4 different departments in the School of Medicine. These researchers are funded by 14 current R01, P01 or R37 NIH grants and are studying systems including cultured cells, C. elegans, and zebrafish. All of these projects involve live-cell imaging, and would benefit significantly from the improved flexibility in excitation and emission wavelengths offered by the proposed instrument.

描述（由申请人提供）：生物标本的成像已成为生物医学科学中越来越重要的工具，具有成像活标本和多个荧光团的能力尤为重要。我们建议购买Zeiss LSM510共聚焦显微镜系统，该系统将安置在犹他大学医学院的细胞成像设施中。该共识将取代过时的入门级共焦（Olympus FVX），并通过添加广泛的激发波长选择以及在多个发射过滤器之间快速切换来提高设施的功能。环境控制的标本室和电动X-Y阶段将特别促进活细胞成像实验。目前，犹他大学的任何地方都没有具有这套功能的乐器。 核心细胞成像设施位于医学院中心，由全职博士学位。具有博士后经验的导演。在他的指导下，该设施非常成功，并大量使用了现有的共聚焦显微镜（> 35小时/周/显微镜）。除了维护显微镜并管理其使用外，导演向用户提供培训和支持。此外，每年他与主要研究人员一起教授教学显微镜课程。对于提供核心显微镜设施的机构承诺有很强的承诺。犹他大学为设施总监的工资提供了全部资金，并保证了服务合同的成本。 该工具的使用将由监督委员会监控，该委员会将与监督所有医学院核心设施的委员会联系。 该工具将主要为医学院4个不同部门的7个主要用户提供服务。这些研究人员由14个当前R01，P01或R37 NIH赠款资助，并正在研究包括培养细胞，秀丽隐杆线虫和斑马鱼在内的系统。所有这些项目都涉及实时成像，并且将从提高的激发和发射波长灵活性中受益匪浅。 建议的仪器。