Ascidian Stock Center (ASC)

海鞘库存中心 (ASC)

• 批准号: 7139253
• 负责人: William Smith
• 金额: $ 34.83万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA BARBARA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7139253
• 关键词: Tunicata; alternatives to animals in research; animal breeding; animal colony; animal genetic material tag; biotechnology; cell bank /registry; genetic manipulation; genetic models; genetic registry /resource /referral center; genetic strain; genetically modified animals; green fluorescent proteins; invertebrate embryology; model design /development; sperm

DESCRIPTION (provided by applicant): The goal of this proposal is the creation of the Ascidian Stock Center (ASC). Ascidians are an emerging model system for the study of embryology, gene regulatory networks, chordate evolution and comparative physiology. Ascidians have great potential as a model system, but they present unique experimental challenges. One of the limitations of ascidian model is the dependence of most of the ascidian community on wild-caught animals. While several species are studied, ascidians of the Ciona genus (C. intestinalis and C. savignyi) are becoming the most widely used, largely due to their wide spread distribution in the wild. The dependence of most laboratories on wild-caught animals means that supplies are unreliable, and sometimes unattainable, and that the types of experiments that can be done are limited. We have established an ascidian culturing facility at the marine laboratory of the University of California at Santa Barbara (UCSB). The Specific Aims of this application propose an expansion of the UCSB facility to accommodate a stock center that will service the growing ascidian community. The purpose of the stock center are two-fold: 1) provide a supplemental source of Ciona adults for research laboratories; and 2) generate and distribute a set of stable transgenic animals that carry tissue-specific enhancers that drive GFP.

描述（由申请人提供）：该提案的目的是创建海外储备中心（ASC）。海沿岸是一种用于研究胚胎学，基因调节网络，弦氏进化和比较生理学的新兴模型系统。海沿作为模型系统具有很大的潜力，但他们提出了独特的实验挑战。海岸模型的局限性之一是大多数西昔日社区对野生动物的依赖。虽然研究了几种物种，但西奥纳属（C. intestinalis and C. savignyi）的海沿岸正在成为最广泛使用的物种，这在很大程度上是由于它们在野外的广泛分布。大多数实验室对野生动物的依赖性意味着供应是不可靠的，有时是无法实现的，并且可以进行的实验类型受到限制。我们已经在加利福尼亚大学圣塔芭芭拉分校（UCSB）的海洋实验室（UCSB）建立了一个海岸文化设施。该应用程序的具体目的是扩大UCSB设施的扩展，以适应一个将为不断增长的海岸社区提供服务的股票中心。股票中心的目的是两个方面：1）为研究实验室提供了ciona成年人的补充来源； 2）生成和分发一组稳定的转基因动物，这些动物携带驱动GFP的组织特异性增强剂。