BioMarker Analysis from Single Cells using Nanobiosensor

使用纳米生物传感器对单细胞进行生物标志物分析

• 批准号: 7050861
• 负责人: Eric R HENDERSON
• 金额: $ 20.43万
• 依托单位: BIOFORCE NANOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2008-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7050861
• 关键词: actins; antigen antibody reaction; bioengineering /biomedical engineering; biomarker; biomedical equipment development; biosensor device; cell sorting; cytology; enzyme linked immunosorbent assay; interleukin 2; intracellular; microarray technology; molecular probes; nanotechnology; photomicrography; printing; proteomics; secretory protein; serine threonine protein kinase; tumor necrosis factor alpha

DESCRIPTION (provided by applicant): The purpose of this Phase I feasibility study to design, construct and evaluate an ultraminiaturized sensor device capable of detect ng protein biomarkers from a few cells and, ultimately, single cells. The device, termed the nanobiosensor, consists of an array of ultraminiaturized protein captur e (antibody) domains printed on a micro fabricated silicon structure. The nanobiosensor combines concepts, methods and instrumentation from nanotechnology and cel I and molecular biology to create a pathway to single-cell proteomic analysis. The nanobiosensor will be used for monitoring both secreted and intracellular protein expression in model cell ular systems. The design of the nanobiosensor allow s the test to be brought directly to the sample origin (in this case, a cell), eliminating the difficulties associated with transporting extremely small quantities of sample extracts to a remote test site. The pr opposed research is enabled by "NanoArrayer" technology developed by the applicant institution for the construction of biochips w itch ultramicro (1-20 mm) to submicron (250 nm) spatial addresses (corresponding to 1/1000th - 1/10,000th of the surface area required for conventional microar rays tests) and requiring only extremely small sample volumes (sub-microliter to picoliter). The nanobiosensor will permit analysis of protein profiles from samples that previously could only be analyzed via PCR amplification of nucleic acids. The proposed nanobiosensor opens the door to fast and infor mative analysis of minute protein samples such as those found in forensics s ituations, neonates and tissue subpopulations (e.g., laser cell microdissected biopsy samples). This level of miniaturization translates to minimally invasive yet data-rich molecular diagnostics for early detection of cancer and other diseases with a significant reduction in patient discomfort.

描述（由申请人提供）：本I阶段可行性研究的目的是设计，构建和评估能够检测几个细胞以及最终单个单元格的NG蛋白生物标志物的超显化传感器设备。该设备称为纳米传感器，由一系列超显微剂化的蛋白质捕获E（抗体）结构域组成。纳米刺传中，纳米技术和CEL I和分子生物学结合了概念，方法和仪器，从而创造了单细胞蛋白质组学分析的途径。纳米传感器将用于监测模型细胞ULAR系统中的分泌和细胞内蛋白表达。纳米传感器的设计使得测试可以直接带到样品起源（在这种情况下为一个单元格），从而消除了与将大量样品提取物运输到远程测试站点相关的困难。 PR反对的研究是由申请人机构开发的“纳米阵列”技术来构建生物芯片，用于建造iTch Ultramicro（1-20毫米）到亚s子（250 nm）空间地址（对应于1/1000th-1/1000th-1/10,000的表面积所需的传统微型射线测试所需的表面积和次数非常小的图片（对应于unsiels smill Simple）和少量的图片（相应）。纳米传感器将允许分析来自以前只能通过核酸的PCR扩增来分析样品的蛋白质谱。提出的纳米传感器为快速和融合分析的分析蛋白样品（例如在取证迭代，新生儿和组织亚群（例如，激光细胞微解剖活检样品）中发现的纳米传感器打开了大门。这种微型化水平转化为最初侵入性但富含数据的分子诊断，以早期检测到癌症和其他疾病，并显着减少患者不适。