Microfabricated Deposition Tools for Creating NanoArrays

用于创建纳米阵列的微加工沉积工具

• 批准号: 7107512
• 负责人: Eric R HENDERSON
• 金额: $ 40万
• 依托单位: BIOFORCE NANOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-02-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7107512
• 关键词: biomaterial development /preparation; biotechnology; method development; miniature biomedical equipment; nanotechnology

DESCRIPTION (provided by applicant): The goal of this SBIR Phase II proposal is to continue and expand the development of microfabricated surface patterning tools (SPTs) for the construction of ultra miniaturized biomaterial arrays (nanoarrays). SPTs can be configured as either pre-loaded molecular ink cartridges analogous to those used in desktop printers, or as an ultraminiaturized version of the pin tool print head in a conventional microarr ayer. SPTs are a consumable component of an instrument we have constructed called NanoArrayer (with support from NIH R44 EB00316). They will also be configured to be compatible with commercial atomic force microscopes. In the proposed Phase II program we will focus on increasing the number of individual spots c reated in a single print cycle while maintaining a high degree of r eproducibility and minimal sample cross contamination. We will optimize linear array (1-D) SPTs containing 10 individually loaded cantilevers and then expand in a second dimension, culminating i n a planar 2-D print head for simultaneous printing of up to 100 nanoarray spots, each capable of containing a unique biomolecular species. E mphasis will be given to controlling sample spil lover (cross-contamination), spot size, printing repr oducibility and SPT-surface alignment. We will also begin to explore forward looking development in the areas of localized magnetic field cantilever control, alternative (non-microcantilever) SPT designs and soft polymer print substrates that can be used for microfluidics and for nanoar ray cloning via contact printing. At the end of the Phase II program we will have an ensemble of STP-based solutions for a variety of ultramicro- and nanoarray applications. Our commer cialization plan will continue throughout the Phase II development program with introduction of improved SPTs as they emerge from the R&D process.

描述（由申请人提供）：该SBIR II期提案的目标是继续并扩大微型化表面图案工具（SPT）的开发，用于构建超小型生物材料阵列（Nanoarrays）。 SPT可以被配置为类似于台式打印机中使用的分子墨盒的预加载的分子墨盒，也可以将其配置为常规微型AYER中的PIN工具打印头的超显微剂化版本。 SPT是我们构建的名为NanoArrayer的仪器（在NIH R44 EB00316的支持下）的易于销量组件。它们还将配置为与商业原子力显微镜兼容。在拟议的II阶段计划中，我们将集中精力增加单个打印周期中重新列出的单个点的数量，同时保持高度的R r效率和最小的样品交叉污染。我们将优化包含10个单独加载的悬臂的线性阵列（1-D）SPT，然后在第二个维度上扩展，最终在平面2-D打印头中使用，以同时打印多达100个纳米阵列，每个点都能包含一个独特的生物分子物种。将对控制样品Spil爱好者（交叉污染），点大小，打印可不可分性和SPT表面对齐。我们还将开始探索局部磁场悬臂控制，替代性（非微型中心）SPT设计和软聚合物打印底物的前瞻性开发，可用于微流体和纳米射线克隆通过接触印刷。在II阶段计划的结尾，我们将拥有一组基于STP的解决方案，用于各种超大型和纳米阵列应用程序。在整个II阶段开发计划中，我们的商业化计划将继续进行，并在研发过程中引入改进的SPT。