Development of an ex vivo buccal mucosal model for understanding the biology of oral health

开发离体颊粘膜模型以了解口腔健康的生物学

• 批准号: 2747060
• 金额: --
• 依托单位: University College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2747060
• 关键词: Development; ex; vivo; buccal; mucosal

In this project it is proposed to collect porcine oral mucosal tissue as a by-product of the food industry and in a similar manner as for skin, develop a robust ex vivo oral mucosal model1-3 that maintains healthy buccal tissue over an extended period suitable for investigating oral healthcare materials. The approach to be taken here will involve: (i) Collection of porcine oral mucosal tissue (ii) Identification of suitable media to maintain the viability of the tissue (iii) Confirmation of barrier function properties of the model (iv) Assessment of tissue biomarkers of inflammation and oxidative stress. In the longer term the model should allow the study of biomarkers of inflammation and oxidative stress in oral tissues and new insights into how the oral mucosal cavity responds to application of specific formulations. Ultimately the project should delivery new information concerning strategies to promote and maintain barrier function and support oral health. This project will establish new models of the oral epithelium for rapid development and screening of formulations for management of oral health and will be of considerable interest to academia and industry.

在这个项目中，建议以与皮肤相似的方式收集猪口服粘膜组织，作为食品工业的副产品，开发出强大的稳健的口服粘膜模型1-3用于调查口腔医疗材料。此处要采取的方法将涉及：（i）收集猪口腔粘膜组织（ii）合适的培养基以维持组织（III）确认模型（IV）评估组织生物标志物的屏障功能特性的可行性（III）炎症和氧化应激。从长远来看，该模型应允许研究口腔组织中炎症和氧化应激的生物标志物，以及对口腔粘膜腔如何响应特定配方的应用的新见解。最终，该项目应提供有关促进和维持障碍功能和支持口腔健康的策略的新信息。 该项目将建立口头上皮的新模型，用于快速开发和筛查口腔健康管理的配方，并将引起学术界和行业的极大兴趣。