3D Digital Modeling of the Drosphila Brain

果蝇大脑的 3D 数字建模

• 批准号: 7010488
• 负责人: VOLKER HARTENSTEIN
• 金额: $ 27.04万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7010488
• 关键词: Drosophilidae; Internet; bioimaging /biomedical imaging; brain morphology; clone cells; computational neuroscience; computer program /software; computer simulation; confocal scanning microscopy; developmental genetics; developmental neurobiology; digital imaging; gene expression; genetic mapping; genetic registry /resource /referral center; metamorphosis; model design /development; nerve stem cell; neurogenesis; neurogenetics; neuropil; nonmammalian vertebrate embryology; phenotype; publications; three dimensional imaging /topography

DESCRIPTION (provided by applicant): Digital 3D models play an increasingly important role in neuroscience. Representing three-dimensional scaffolds in which functional data and gene expression data are entered and displayed graphically, the digital models become analytical tools that allow one to address neural connectivity and function, as well as gene function and gene interactions. This grant application proposes to generate a series of standardized digital atlas models of the developing Drosophila brain, a system used by many to investigate the genetic mechanism controlling the formation and function of neuronal circuits. The fly brain is formed by an invariant set of neuroblast lineages which represent structural units in terms of cell body location, axonal projection, and (to an extent that will be addressed in this proposal) connectivity. Axonal and dendritic arborizations, establish morphologically distinct neuropile compartments that are visible from the late embryo towards the adult. Compartments and lineages form a stereotyped pattern that will be captured in the proposed digital models. Having in mind their usefulness for us and others, as well as feasibility, the following models are proposed: (1) early embryonic neuroblast map, (2) late embryonic primary lineages in relation to evolving neuropile, (3) late larval secondary lineages in relation to neuropile compartments, (4) evolving secondary tract systems and neuropile compartments of the pupa. These models represent an integrated series because the "genetic address" of each neuroblast, defined by the known sets of genes expressed in the early embryonic neuroblast map, will be linked to the population of neurons and their axons modeled for the late embryo, larva and pupa. The goal of this modeling project is to provide a tool shared with the community, allowing to exploit the Drosphila brain more efficiently for developmental-genetic and functional questions. Models of neural lineages will make it possible to phrase specific experiments and to interpret mutant phenotypes.

描述（由申请人提供）：数字 3D 模型在神经科学中发挥着越来越重要的作用。 数字模型代表三维支架，其中功能数据和基因表达数据以图形方式输入和显示，成为分析工具，使人们能够解决神经连接和功能，以及基因功能和基因相互作用。 该拨款申请旨在生成一系列正在发育的果蝇大脑的标准化数字图谱模型，该系统被许多人用来研究控制神经元回路形成和功能的遗传机制。 果蝇大脑由一组不变的神经母细胞谱系形成，这些谱系代表细胞体位置、轴突投影和（在本提案中将解决的一定程度上）连接性的结构单元。 轴突和树突状树枝化建立了形态上不同的神经丛隔室，从晚期胚胎到成年都可见。 区室和谱系形成了一种刻板模式，将在提议的数字模型中捕获。 考虑到它们对我们和其他人的有用性以及可行性，提出了以下模型：（1）早期胚胎神经母细胞图，（2）与进化神经桩相关的晚期胚胎初级谱系，（3）晚期幼虫次级谱系与神经桩隔室的关系，（4）进化的次级道系统和蛹的神经桩隔室。 这些模型代表了一个完整的系列，因为每个神经母细胞的“遗传地址”（由早期胚胎神经母细胞图中表达的已知基因组定义）将与为晚期胚胎、幼虫和幼虫建模的神经元群体及其轴突联系起来。蛹。 该建模项目的目标是提供一个与社区共享的工具，允许更有效地利用果蝇大脑来解决发育遗传和功能问题。 神经谱系模型将使表达特定实验和解释突变表型成为可能。