3D Digital Modeling of the Drosphila Brain

果蝇大脑的 3D 数字建模

• 批准号: 7010488
• 负责人: VOLKER HARTENSTEIN
• 金额: $ 27.04万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-02-01 至 2010-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7010488
• 关键词: Drosophilidae; Internet; bioimaging /biomedical imaging; brain morphology; clone cells; computational neuroscience; computer program /software; computer simulation; confocal scanning microscopy; developmental genetics; developmental neurobiology; digital imaging; gene expression; genetic mapping; genetic registry /resource /referral center; metamorphosis; model design /development; nerve stem cell; neurogenesis; neurogenetics; neuropil; nonmammalian vertebrate embryology; phenotype; publications; three dimensional imaging /topography

DESCRIPTION (provided by applicant): Digital 3D models play an increasingly important role in neuroscience. Representing three-dimensional scaffolds in which functional data and gene expression data are entered and displayed graphically, the digital models become analytical tools that allow one to address neural connectivity and function, as well as gene function and gene interactions. This grant application proposes to generate a series of standardized digital atlas models of the developing Drosophila brain, a system used by many to investigate the genetic mechanism controlling the formation and function of neuronal circuits. The fly brain is formed by an invariant set of neuroblast lineages which represent structural units in terms of cell body location, axonal projection, and (to an extent that will be addressed in this proposal) connectivity. Axonal and dendritic arborizations, establish morphologically distinct neuropile compartments that are visible from the late embryo towards the adult. Compartments and lineages form a stereotyped pattern that will be captured in the proposed digital models. Having in mind their usefulness for us and others, as well as feasibility, the following models are proposed: (1) early embryonic neuroblast map, (2) late embryonic primary lineages in relation to evolving neuropile, (3) late larval secondary lineages in relation to neuropile compartments, (4) evolving secondary tract systems and neuropile compartments of the pupa. These models represent an integrated series because the "genetic address" of each neuroblast, defined by the known sets of genes expressed in the early embryonic neuroblast map, will be linked to the population of neurons and their axons modeled for the late embryo, larva and pupa. The goal of this modeling project is to provide a tool shared with the community, allowing to exploit the Drosphila brain more efficiently for developmental-genetic and functional questions. Models of neural lineages will make it possible to phrase specific experiments and to interpret mutant phenotypes.

描述（由申请人提供）：数字3D模型在神经科学中起着越来越重要的作用。 代表三维脚手架，其中功能数据和基因表达数据被以图形方式显示，数字模型成为分析工具，可以解决神经连接性和功能，以及基因功能和基因相互作用。 该赠款应用建议生成一系列发展果蝇大脑的标准化数字图集模型，该系统用于研究控制神经元电路的形成和功能的遗传机制。 苍蝇大脑是由一组不变的神经细胞谱系形成的，这些神经细胞谱系代表了细胞体位置，轴突投影，并且（在此提案中将解决的程度）连通性。 轴突和树突状的植物，建立形态上不同的神经隔室，从晚期胚胎向成人可见。 隔室和血统形成了刻板印象模式，该模式将在拟议的数字模型中捕获。 Having in mind their usefulness for us and others, as well as feasibility, the following models are proposed: (1) early embryonic neuroblast map, (2) late embryonic primary lineages in relation to evolving neuropile, (3) late larval secondary lineages in relation to neuropile compartments, (4) evolving secondary tract systems and neuropile compartments of the pupa. 这些模型代表了一个集成的序列，因为每个神经细胞的“遗传地址”（由早期胚胎神经细胞图中表达的已知基因集定义）将与为后期胚胎，larva和pupa建模的神经元及其轴突的群体有关。 该建模项目的目的是提供与社区共享的工具，从而使Raflosphila大脑更有效地用于发育遗传和功能性问题。 神经谱系的模型将使特定的实验和解释突变表型成为可能。