CORE-- MICROSCOPE FACILITY

核心——显微镜设施

• 批准号: 7090680
• 负责人: LESLIE P TOLBERT
• 金额: $ 17.85万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7090680
• 关键词: bioimaging /biomedical imaging; biomedical facility; charge coupled device camera; computer data analysis; confocal scanning microscopy; developmental neurobiology; electron microscopy; imaging /visualization /scanning; time resolved data; video microscopy

DESCRIPTION: The Confocal and Electron Microscopy Facility is equipped for electron microscopy (EM) and light microscopy (LM) including confocal and live-cell imaging, including time-lapse video and calcium imaging systems. It has also been expanded during the previous funding period to include a Computer Imaging Facility for image processing and analysis. All 5 projects will use the LM-based systems and 3 will use EM. In addition to the equipment, the facility provides training and technical support. The facility is overseen by Dr. Leslie Tolbert and employs three staff members: 1) a Master's level Senior Research Specialist, Ms. Jansma, (60% effort on PPG and 40% for the Division) technically expert in EM and confocal microscopy, she maintains all of the scopes, trains new users and helps to develop protocols. 2) a part-time research technician, Ms. Potts (20% effort here and 80% effort on Project 2) provides training and assistance with the confocal and live-cell systems. 3) a part-time computer expert, Mr. Yuhas (75% effort on PPG and 25% effort in the Division) maintains computers, installs upgrades and trains users in software.

描述：共焦和电子显微镜设备配备用于 电子显微镜 (EM) 和光学显微镜 (LM)，包括共焦和 活细胞成像，包括延时视频和钙成像系统。它 在上一个资助期间也得到了扩展，包括计算机 用于图像处理和分析的成像设施。所有 5 个项目都将使用 基于 LM 的系统和 3 将使用 EM。除了设备，还有设施 提供培训和技术支持。该设施由 Leslie 博士负责监督 Tolbert 并雇用了三名工作人员：1) 硕士级别的高级研究人员 专家 Jansma 女士（60% 的精力用于 PPG，40% 的精力用于该部门） 作为 EM 和共焦显微镜技术专家，她保留了所有 范围、培训新用户并帮助开发协议。 2）兼职 研究技术员，Potts 女士（此处 20% 的努力，项目 2 的 80% 的努力） 提供共聚焦和活细胞系统的培训和帮助。 3）一个 兼职计算机专家 Yuhas 先生（75% 的精力在 PPG 上，25% 的精力在 部门）维护计算机、安装升级并对用户进行软件培训。