CORE-- MICROSCOPE FACILITY

核心——显微镜设施

• 批准号: 6665764
• 负责人: LESLIE P TOLBERT
• 金额: $ 23.19万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6665764
• 关键词: bioimaging /biomedical imaging; biomedical facility; charge coupled device camera; computer data analysis; confocal scanning microscopy; developmental neurobiology; electron microscopy; imaging /visualization /scanning; time resolved data; video microscopy

DESCRIPTION: The Confocal and Electron Microscopy Facility is equipped for electron microscopy (EM) and light microscopy (LM) including confocal and live-cell imaging, including time-lapse video and calcium imaging systems. It has also been expanded during the previous funding period to include a Computer Imaging Facility for image processing and analysis. All 5 projects will use the LM-based systems and 3 will use EM. In addition to the equipment, the facility provides training and technical support. The facility is overseen by Dr. Leslie Tolbert and employs three staff members: 1) a Master's level Senior Research Specialist, Ms. Jansma, (60% effort on PPG and 40% for the Division) technically expert in EM and confocal microscopy, she maintains all of the scopes, trains new users and helps to develop protocols. 2) a part-time research technician, Ms. Potts (20% effort here and 80% effort on Project 2) provides training and assistance with the confocal and live-cell systems. 3) a part-time computer expert, Mr. Yuhas (75% effort on PPG and 25% effort in the Division) maintains computers, installs upgrades and trains users in software.

描述：共聚焦和电子显微镜设施配备了 电子显微镜（EM）和光学显微镜（LM），包括共焦和 活细胞成像，包括延时视频和钙成像系统。它 在上一个资金期间也已扩展到包括计算机 图像处理和分析的成像设施。所有5个项目都将使用 基于LM的系统和3将使用EM。除设备外，设施 提供培训和技术支持。该设施由莱斯利博士监督 托尔伯特并雇用三名员工：1）硕士级高级研究 专家Jansma女士（PPG上的努力为60％，该部门40％） 在EM和共聚焦显微镜方面的技术专家，她保持了所有 示波器，训练新用户并有助于制定协议。 2）兼职 研究技术员，Potts女士（这里有20％的努力，项目2的努力为80％） 为共聚焦和活细胞系统提供培训和帮助。 3）a 兼职计算机专家Yuhas先生（PPG上的努力为75％，努力为25％ 部门维护计算机，安装升级并在软件中培训用户。