Core-Analytical and Radioimmunoassay

核心分析和放射免疫分析

• 批准号: 7062945
• 负责人: BARBARA D ALEXANDER
• 金额: $ 20.47万
• 依托单位: UNIVERSITY OF MISSISSIPPI MED CTR
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-12-01 至 2008-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7062945
• 关键词: RNase protection assay; analytical method; animal tissue; biohazard control; biomedical facility; clinical research; enzyme linked immunosorbent assay; genotype; hemodynamics; high performance liquid chromatography; histology; image processing; immunocytochemistry; in situ hybridization; microarray technology; molecular biology; morphometry; nucleic acid quantitation /detection; protein quantitation /detection; radioimmunoassay; sample collection; sectioning

The Analytical and Radioimmunoassay Core will provide support for radioimmunoassays, enzyme-linked immunoassays (ELISA), HPLC, various molecular and chemical analyses, and histological studies. The core will provide assay results for a large number of hormones and cytokines as well as maintenance and supervision of key equipment in a central facility. The core will also provide tissue sections from paraffin embedded tissues, plastic embedded tissues, and frozen tissues for morphometric and stereological analyses. The morphometric and stereological measurements will be used in conjunction with immunohistochemistry to achieve the goals of the various projects. In addition, the molecular core will provide assistance with quantitation of RNA and protein, gene array, and genotyping.

分析和放射免疫分析核心将为放射免疫分析、酶联免疫分析 (ELISA)、HPLC、各种分子和化学分析以及组织学研究提供支持。该核心将提供大量激素和细胞因子的检测结果，以及中央设施中关键设备的维护和监督。该核心还将提供石蜡包埋组织、塑料包埋组织和冷冻组织的组织切片，用于形态测量和体视学分析。形态测量和体视学测量将与免疫组织化学结合使用，以实现各个项目的目标。此外，分子核心还将为 RNA 和蛋白质定量、基因阵列和基因分型提供帮助。