Extracellular Matrix of Bacillus subtilis Biofilms

枯草芽孢杆菌生物膜的细胞外基质

• 批准号: 6958550
• 负责人: Ashlee Miriam Earl
• 金额: $ 4.88万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-12-01 至 2007-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6958550
• 关键词: Bacillus subtilis; bacterial genetics; bacterial proteins; beta galactosidase; biofilm; chromatin immunoprecipitation; enzyme activity; extracellular matrix; gel mobility shift assay; gene expression; gene mutation; genetic regulation; genetic screening; microarray technology; operon; postdoctoral investigator; transposon /insertion element

DESCRIPTION (provided by applicant): It is now widely recognized that bacteria have the widespread capacity to form surface-associated multicellular aggregates, commonly refered to as "biofilms". When organisms, be it eukaryotes or prokaryotes, transition from unicellularity to multicellularity they produce an extracellular matrix that provides structural support and facilitates surface attachment. Within biofilms the constituent cells are embedded in matrices composed of diverse extracellular polymeric substances. Although the process of biofilm formation appears to be dependent upon the formation of an extracellular matrix, the content, regulation and precise function of these exported products are still largely unknown. Analysis of the B. subtilis genome revealed the presence of a cluster of genes, the yve genes, whose products are predicted to be involved in the biosynthesis of a possible extracellular polysaccharide. Experimentally, I will focus on characterizing the molecular mechanism of the spatio-temporal regulation of the yve operon and I will identify additional genes involved in matrix production in Bacillus subtilis biofilms.

描述（由申请人提供）：现在人们广泛认识到细菌具有形成表面相关多细胞聚集体（通常称为“生物膜”）的广泛能力。当生物体，无论是真核生物还是原核生物，从单细胞转变为多细胞时，它们会产生细胞外基质，提供结构支撑并促进表面附着。在生物膜内，组成细胞嵌入由多种细胞外聚合物质组成的基质中。尽管生物膜的形成过程似乎依赖于细胞外基质的形成，但这些出口产品的含量、调节和精确功能仍然很大程度上未知。对枯草芽孢杆菌基因组的分析揭示了一组基因的存在，即 yve 基因，其产物预计参与可能的细胞外多糖的生物合成。在实验上，我将重点描述 yve 操纵子时空调节的分子机制，并将确定参与枯草芽孢杆菌生物膜中基质生产的其他基因。