Characterization of candidate histone methyltransferases

候选组蛋白甲基转移酶的表征

• 批准号: 7064507
• 负责人: Michael Lee
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7064507
• 关键词: binding proteins; gene expression; genetically modified animals; histones; laboratory mouse; methyltransferase; posttranscriptional RNA processing; protein structure function; yeast two hybrid system

We conducted a comprehensive analysis of transcription of two candidate histone methyltransferases in human and mouse tissues, showing tissue-specific expression of alternatively spliced and read-through transcripts. We conducted several yeast two-hybrid screens to identify protein-binding partners for the putative histone methyltransferases. These screens yielded several intriguing candidates that are being characterized further by biochemical and genetic methods. We have constructed a conditional knockout plasmid to generate a knockout mouse model lacking one of the putative histone methyltransferases located on a frequently deleted chromosomal region.

我们对人类和小鼠组织中两种候选组蛋白甲基转移酶的转录进行了全面分析，显示了选择性剪接和通读转录本的组织特异性表达。我们进行了几次酵母双杂交筛选，以确定假定的组蛋白甲基转移酶的蛋白质结合伴侣。这些筛选产生了几种有趣的候选物，正在通过生化和遗传方法进一步表征。我们构建了一个条件敲除质粒，以产生一种敲除小鼠模型，该模型缺乏位于经常缺失的染色体区域上的一种假定的组蛋白甲基转移酶。