Gene Expression Profiling of Normal Tiss

正常 Tiss 的基因表达谱

• 批准号: 7062373
• 负责人: Javed KHAN
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7062373
• 关键词: Internet; data collection; gene expression profiling; gene targeting; human tissue; information dissemination; neoplasm /cancer genetics; neoplasm /cancer immunotherapy; pediatric neoplasm /cancer; postmortem; publications; therapy adverse effect; tissue resource /registry; tumor antigens

The non-specific nature of current cancer therapeutics, the lack of response in high-risk disease, and the severe, sometimes fatal, organ toxicities have driven cancer therapeutics towards rationally designed and specific molecularly targeted therapy. It is hoped that targeting genes expressed only in cancer, and not in normal tissue will minimize the side effects of therapy. These unique genes can also be used as tumor antigens for the development of vaccine-based immune therapies for childhood cancer (a primary interest of our branch of Pediatric Oncology Branch, NCI), which will reduce the risk of serious autoimmune manifestations. To achieve this aim, we are performing gene expression analysis on pediatric normal organ samples. The tissues were obtained from the Maryland Brain and Tissue Bank (http://medschool.umaryland.edu/btbank/). These samples include tissues from cerebrum, cerebellum, heart, lung, liver, pancreas, stomach, small intestine, large intestine, kidney, spleen, skeletal muscle, ovary, testes, ureter, uterus, bladder, adrenal gland, and prostate. The median age of the subjects from which the samples were obtained is 19yrs (range 2-40yrs), with a median post mortem interval (PMI) of 11hrs (range 4-19hrs). Information from the Bank suggests that the RNAs obtained from tissues within these PMIs are of good quality as judged by integrity of the ribosomal bands. The data generated by gene expression profiling will be collated and compared with the data generated from the malignant tissues. After publication, we will make the data available to outside investigators for searching via a web-based format.This data will be of utility for developing rationally designed molecularly targeted therapeutics in diseases such as cancer, as well as for exploring the functions of genes.

当前癌症治疗剂的非特异性，高风险疾病中缺乏反应以及严重的，有时是致命的器官毒性使癌症治疗方法促进了理性设计和特定的分子靶向疗法。希望靶向仅在癌症中表达的基因，而不是在正常组织中，可以最大程度地减少治疗的副作用。这些独特的基因也可以用作肿瘤抗原，用于开发儿童癌症的基于疫苗的免疫疗法（我们的儿科肿瘤科分支NCI的主要兴趣），这将降低严重自身免疫性表现的风险。为了实现这一目标，我们正在对小儿正常器官样本进行基因表达分析。这些组织是从马里兰州脑和组织库（http://medschool.umaryland.edu/btbank/）获得的。这些样本包括来自大脑，小脑，心脏，肺，肝脏，胰腺，胃，小肠，大肠，肾脏，肾脏，脾脏，骨骼肌，骨骼肌肉，卵巢，测试剂，输尿管，子宫，子宫，膀胱，肾上腺，肾上腺，肾上腺和前列腺的组织。获得样品的受试者的中位年龄为19岁（范围2-40岁），验尸后间隔（PMI）为11小时（4-19小时）。银行的信息表明，根据核糖体条带的完整性，从这些PMI中的组织获得的RNA具有良好的质量。基因表达分析产生的数据将进行整理，并将其与来自恶性组织产生的数据进行比较。发表后，我们将向外部研究人员提供数据，以通过基于Web的格式进行搜索。此数据将有效地开发癌症等疾病中的合理设计的分子靶向疗法，以及探索基因功能。