Investigating the utility and exploring biological targets of enhanced micro-wound healing compounds in 2D and 3D skin models

研究增强微伤口愈合化合物在 2D 和 3D 皮肤模型中的效用并探索其生物靶标

• 批准号: 2617469
• 金额: --
• 依托单位: Queen Mary University of London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2617469
• 关键词: Investigating; utility; exploring; biological; targets

Micro-wound healing is a dynamic, physiological process in which keratinocytes transition to an activated, migratory state to re-establish the epidermal barrier and resurface wounds. Although such wounds typically heal, effective intervention could offer enhanced micro-wound healing within mechanistically relevant timeframes. Whilst this is a rapidly evolving area, the successful development of enhanced micro-wound healing compounds (EMH compounds) requires systematic investigation of their utility and exploration of candidate protein targets. An important hurdle is the establishment of a 3D model with highly reproducible micro-wounds and cutting-edge approaches to monitor healing dynamics. We have established 2D and 3D human living skin equivalent (LSEs) models that enable us to perform automated high content image analysis to generate 'multidimensional drug profiles'. In this proposal we integrate our recently established 3D bioprinted models of wound healing to investigate the utility of EMH compounds and explore biological targets of enhanced micro-wound healing.

微伤口愈合是一个动态的生理过程，其中角质形成细胞转变为激活的迁移状态，以重建表皮屏障并重新修复伤口。尽管此类伤口通常会愈合，但有效的干预可以在机械相关的时间范围内增强微伤口的愈合。虽然这是一个快速发展的领域，但增强微伤口愈合化合物（EMH 化合物）的成功开发需要对其效用进行系统研究并探索候选蛋白质靶标。一个重要的障碍是建立具有高度可重复性微伤口的 3D 模型和监测愈合动态的尖端方法。我们已经建立了 2D 和 3D 人类活体皮肤等效 (LSE) 模型，使我们能够执行自动化高内涵图像分析，以生成“多维药物概况”。在本提案中，我们整合了最近建立的伤口愈合 3D 生物打印模型，以研究 EMH 化合物的效用并探索增强微伤口愈合的生物学目标。