Receptor Specifcity in the Proliferation and Differentiation of Stem Cells

干细胞增殖和分化中的受体特异性

• 批准号: 7076455
• 负责人: C. Anthony Blau
• 金额: $ 31.88万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7076455
• 关键词: NOD mouse; SCID mouse; biological signal transduction; biotechnology; cell differentiation; cell proliferation; chemical binding; dogs; embryonic stem cell; fibroblast growth factor; flow cytometry; gene therapy; genetic manipulation; genetically modified animals; growth factor receptors; hematopoiesis; hematopoietic stem cells; hematopoietic tissue transplantation; human embryonic stem cell line; human tissue; laboratory mouse; protein structure function; receptor binding; stem cell transplantation; tissue /cell culture

DESCRIPTION (provided by applicant): The goal of this application is to test a candidate "universal" cell growth switch, a conditional derivative of the fibroblast growth factor receptor 1, F36VFGFR1, in preclinical models of gene and cell therapy. In specific aim 1, we will test whether different growth factor receptor signaling domains differentially regulate human hemopoiesis. In specific aim 2 we will compare the nature, intensity and duration of the hematopoietic response to F36VFGFR1 signaling versus F36Vmp1 signaling in dogs. In specific aim 3 we will test whether F36VFGFR1 signaling promotes self-renewal in engineered human embryonic stem (ES) cells. We will be using the human embryonic stem cell line WA01.

描述（由申请人提供）：本申请的目的是测试候选“通用”细胞生长开关，这是基因和细胞疗法的临床前模型中成纤维细胞生长因子受体1，F36VFGFR1的条件衍生物。在特定目标1中，我们将测试不同生长因子受体信号传导域是否差异化调节人类造血。在特定目标2中，我们将比较造血反应的性质，强度和持续时间与F36VFGFR1信号传导与狗中的F36VMP1信号。在特定目标3中，我们将测试F36VFGFR1信号传导是否促进了工程人类胚胎茎（ES）细胞中的自我更新。我们将使用人类胚胎干细胞系WA01。