Cell Biosystems Firefly 3000 system

Cell Biosystems Firefly 3000 系统

基本信息

批准号：
7792826
负责人：
C. Anthony Blau
金额：
$ 31.84万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-06-15 至 2011-06-14
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7792826
关键词：
Address Cell Count Cell Separation Cells Commit Core Facility Fees Fireflies Fred Hutchinson Cancer Research Center Funding Housing Laboratories Maintenance Pediatric Hospitals Population Protein Analysis Protein Isoforms Research Research Infrastructure Sample Size Sampling Signal Transduction Signaling Protein System Universities Washington instrument interest laser capture microdissection operation public health relevance

Address Cell Count Cell Separation Cells Commit Core Facility Fees Fireflies Fred Hutchinson Cancer Research Center Funding Housing Laboratories Maintenance Pediatric Hospitals Population Protein Analysis Protein Isoforms Research Research Infrastructure Sample Size Sampling Signal Transduction Signaling Protein System Universities Washington instrument interest laser capture microdissection operation public health relevance

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项目摘要

DESCRIPTION (provided by applicant): We request funds to support the purchase an instrument that will help more than 19 different laboratories at the University of Washington (UW), Seattle Children's Hospital, and the Fred Hutchinson Cancer Research Center (FHCRC) address critical barriers in their research. The Firefly 3000 Protein Analysis System (Cell Biosciences, Palo Alto, CA) allows all isoforms of a signaling protein of interest to be precisely quantified using sample sizes containing as few as 25 cells. The ability to quantitatively assess cell signaling using very low cell numbers opens the door to assessing signal transduction in rare cell populations obtained after cell sorting or after laser capture microdissection. This instrument will be housed in our Cell Analysis Core Facility at the UW's South Lake Union Campus, using the same infrastructure that supports operation of the Core. In addition, the UW has committed a total of $120,000 over 5 years to support the maintenance of the instrument, assuring that user fees can be kept at relatively low levels. PUBLIC HEALTH RELEVANCE: This application seeks funding for the Firefly 3000 Protein Analysis System. This instrument will support more than 19 labs at the UW, FHCRC and Seattle Children's Hospital, allowing signal transduction to be quantitatively assessed using samples of as few as 25 cells.

描述（由申请人提供）：我们要求资金支持购买工具，该工具将帮助华盛顿大学（UW），西雅图儿童医院和弗雷德·哈钦森癌症研究中心（FHCRC）的19个不同实验室，以解决其研究中的关键障碍。 Firefly 3000蛋白质分析系统（Cell Biosciences，Palo Alto，CA）允许使用含量较少25个细胞的样本量来精确量化感兴趣的信号蛋白的所有同工型。使用非常低的细胞数量来定量评估细胞信号传导的能力为评估细胞分类后稀有细胞种群或激光捕获捕获显微解剖后获得的信号转导能力打开了大门。该仪器将使用支持核心操作的相同基础设施，将其安置在西澳大学南湖联合校园的细胞分析核心设施中。此外，UW在5年内总共承诺了120,000美元以支持该工具的维护，并确保可以将用户费用保持在相对较低的水平。公共卫生相关性：该应用程序寻求用于Firefly 3000蛋白质分析系统的资金。该仪器将在UW，FHCRC和西雅图儿童医院支持19个以上的实验室，从而可以使用较少25个细胞的样品进行定量评估信号转导。