Regulation of Embryonic Stem Cell Self-Renewal

胚胎干细胞自我更新的调控

• 批准号: 8598889
• 负责人: C. Anthony Blau
• 金额: $ 35.93万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-12-01 至 2017-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8598889
• 关键词: Adopted; Affect; Animals; Antibodies; Biological Assay; Blood capillaries; Butyrates; Cardiac Myocytes; Cells; Characteristics; Co-Immunoprecipitations; Collaborations; Commit; Computer Analysis; Confocal Microscopy; Diapause; Distant; Embryo; Environment; Epiblast; Epigenetic Process; Event; Foundations; Functional RNA; Funding; Genes; Goals; Histone Deacetylase Inhibitor; Human; Immunofluorescence Immunologic; In Vitro; Inner Cell Mass; Isoelectric Focusing; Knockout Mice; Learning; Light; Mass Spectrum Analysis; Messenger RNA; Metabolic; MicroRNAs; Modeling; Modification; Molecular; Mus; Organism; Pathway interactions; Phosphorylation; Physiological; Pluripotent Stem Cells; Post-Translational Protein Processing; Process; Protein Isoforms; Proteins; RNA Sequences; Regulation; STK11 gene; Signal Pathway; Signaling Protein; Starvation; Stem cells; Stress; Techniques; Testing; Tissues; animation; base; cancer stem cell; capillary; embryonic stem cell; human embryonic stem cell; implantation; in vitro Model; in vivo; in vivo Model; instrument; interest; member; natural Blastocyst Implantation; notch protein; novel; pluripotency; preimplantation; progenitor; response; self-renewal; succinyl-trialanine-4-nitroanilide; transcriptome sequencing

Project 1 will investigate the molecular basis of stem cell quiescence using the physiological phenomenon known as diapause as a model. We will also test whether similar molecular events occur in human pluripotent stem cells. Specific Aim 1 will use a diapause model to characterize stem cell quiescence in vivo, while Specific Aim 2 will characterize stem cell quiescence using novel in vitro models.

项目 1 将使用滞育这一生理现象作为模型，研究干细胞静止的分子基础。我们还将测试类似的分子事件是否发生在人类多能干细胞中。具体目标 1 将使用滞育模型来表征体内干细胞静止，而具体目标 2 将使用新型体外模型来表征干细胞静止。