CORE--TECHNICAL

核心--技术

• 批准号: 6875427
• 负责人: LAWRENCE D LONGO
• 金额: $ 44.73万
• 依托单位: LOMA LINDA UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6875427
• 关键词: altitude; biomedical facility; embryo /fetus cell /tissue; environmental adaptation; hypoxia; sheep

Pregnant sheep will continue to be used for these studies, as they are readily available, are relatively inexpensive (as compared with primates), the fetuses are large enough so that adequate amounts of the several tissues can be studied (cerebral and pulmonary arteries, pituitary and adrenal gland, et cetera), and we and others have amassed a considerable body of physiologic data on this species. Essentially all of the individual projects in this proposal will use pregnant or nonpregnant sheep which have been acclimatized to high altitude, long-term hypoxemia, or normoxic controls. At the time of sacrifice, tissues from these animals will be partitioned among the several investigators and projects. Thus, this Technical Core includes the sheep for these studies. In addition, it includes the expense for several major items of equipment required for most of these experiments. The experimental animal and equipment technical core will be utilized for essentially every project in common by all members of the Center. Such a technical core optimizes the efficient operation of the laboratories, the experiments per se, and the data acquisition and analysis.

怀孕的绵羊将继续用于这些研究，因为它们很容易获得，相对便宜（与灵长类动物相比），胎儿足够大，因此 可以研究足够数量的几种组织（脑动脉和肺动脉、垂体和肾上腺等），并且我们和其他人已经积累了有关该物种的大量生理数据。基本上，该提案中的所有单个项目都将使用已适应高海拔、长期低氧血症或常氧控制的怀孕或非怀孕绵羊。处死时，这些动物的组织将分配给几个研究人员和项目。因此，该技术核心包括用于这些研究的绵羊。此外，它还包括大多数这些实验所需的几项主要设备的费用。实验动物和设备技术核心将用于中心所有成员共同的几乎每个项目。这样的技术核心优化了实验室、实验本身以及数据采集和分析的高效运行。