Neuropathology and Tissue Culture Core

神经病理学和组织培养核心

基本信息

批准号：
6919799
负责人：
Luis Del Valle
金额：
$ 22.97万
依托单位：
TEMPLE UNIV OF THE COMMONWEALTH
依托单位国家：
美国
项目类别：
财政年份：
2004
资助国家：
美国
起止时间：
2004-12-01 至 2009-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6919799
关键词：
AIDS AIDS /HIV neuropathy AIDS dementia complex Polyomavirus hominis 2 biomedical facility embryo /fetus cell /tissue genetically modified animals human immunodeficiency virus 1 human tissue laboratory mouse laboratory rat neuropathology progressive multifocal leukoencephalopathy tissue /cell culture tissue /cell preparation tissue resource /registry viral myelinopathy virus virus interaction

项目摘要

This proposal seeks continued funding of the Neuropathology and Tissue Culture Core to assist the overall program project grant in evaluating the pathogenesis of HIV-1-associated dementia and Progressive Multifocal Leukoencephalopathy (PML) and the cooperative molecular mechanisms through which HIV-1 and JC virus interact. We will provide neuropathological expertise fore the evaluation of surgical and autopsy brain tissue samples from AIDS and non-AIDS PML patients with HIV-1 encephalopathy and control tissue to complement molecular studies performed in Projects 1,2, and 3. We will provide highly purified cultures of microglia, oligodendrocytes, and astrocytes from human fetal CNS tissue as needed for the individual projects. The cultures will undergo quality control testing to ensure uniform distribution across the projects. We will provide cultures of established permanent cell lines, including the progenitor CG-4 cells and mouse embryo fibroblasts from Pur-alpha knockout mice as a service to all three projects. We will also offer on demand culturing of primary cells or establishment of cell lines from rats and genetically modified mice. The proposed Core services will provide essential and uniform material to the three participant projects to assist in their studies on the molecular biology of HIV-1 and JCV interaction with host cellular factors and will contribute to our understanding of the pathogenesis of these two CNS disorders.

该提案寻求继续资助神经病理学和组织培养核心，以帮助总体计划项目赠款评估HIV-1相关痴呆症的发病机理和进行性多焦点白血病（PML）以及HIV-1和JC病毒相互作用的合作分子机制。 We will provide neuropathological expertise fore the evaluation of surgical and autopsy brain tissue samples from AIDS and non-AIDS PML patients with HIV-1 encephalopathy and control tissue to complement molecular studies performed in Projects 1,2, and 3. We will provide highly purified cultures of microglia, oligodendrocytes, and astrocytes from human fetal CNS tissue as needed for the individual projects.这些文化将经过质量控制测试，以确保整个项目的统一分配。我们将提供已建立的永久细胞系的培养物，包括祖细胞CG-4细胞和小鼠胚胎成纤维细胞中的小鼠成纤维细胞，作为所有三个项目的服务。我们还将提供对原代细胞的需求培养或建立来自大鼠和转基因小鼠的细胞系。拟议的核心服务将为三个参与者项目提供必不可少的材料，以帮助他们研究HIV-1和JCV与宿主细胞因素的分子生物学研究，并将有助于我们理解这两种CNS疾病的发病机理。