CORE--ANIMAL MODEL

核心--动物模型

• 批准号: 6959581
• 负责人: CHARLES O ELSON
• 金额: $ 23.11万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6959581
• 关键词: animal breeding; animal colony; colitis; disease /disorder model; gene expression; genetic manipulation; genetic strain; genetically modified animals; laboratory mouse; model design /development; pathologic process; tissue resource /registry

Animal Model will provide all mice used and it will be responsible for ordering mice, performing the various manipulations required to induce experimental colitis, maintaining a barrier facility, performing routine surveys to ensure absence of pathogens in the colony, and assist in harvesting tissues from such mice. Centralization of these functions provides uniformity and quality control of mice being studied in the various projects, reduces cost, and will help conserve numbers of mice required by ensuring their optimal use in the projects. A second aim is that of centralized pathologic analyses which will be performed by Dr. Trenton Schoeb, an expert veterinary pathologist. Tissue sections from the various projects will be coded prior to interpretation and quantitative scoring where needed. This facilitates interactions among the projects and also the comparison of results. A third purpose is to provide genetically modified transgenic and knockout mouse strains for use in experiments. Such genetically altered strains are powerful analytic tools for defining the role of specific gene products in complex biologic systems, and have become the "reagents" that allow fundamental questions to be experimentally addressed that had been previously unapproachable. Mice with induced mutations frequently have to be bred onto other background strains for optimal study, which requires screening of individual animals in each generation by identification of one or more target genetic elements. Dr. Charles Elson will serve as Director and Dr. Casey Weaver will serve as Co-Director. Mice will be housed under barrier conditions in a new vivarium which is physically linked to the new Shelby Research Building. Dr. Sam Gartner from the Department of Comparative Medicine will serve as Consultant An Oversight Committee consisting of the PI of each project with Drs. Schoeb and Gartner will oversee the usage and function.

动物模型将提供所有使用的小鼠，它将负责订购小鼠，进行诱导实验性结肠炎所需的各种操作，维持屏障设施，进行常规调查以确保菌落中没有病原体并协助帮助 从这种小鼠那里收集组织。这些功能的集中化提供了对各种项目中研究的小鼠的统一性和质量控制，降低成本，并将有助于 通过确保其在项目中的最佳用途来保存所需的小鼠数量。第二个目的是集中的病理分析，该分析将由Trenton Schoeb博士进行。 专家兽医病理学家。各个项目的组织部分将在 需要解释和定量评分。这有助于项目之间的互动以及结果的比较。第三目的是提供转基因的转基因和基因敲除小鼠菌株进行实验。这种遗传变化的菌株是定义特定基因产物在复杂生物学系统中的作用的强大分析工具，并已成为允许基本问题的“试剂”，可以实验解决以前无法接受的问题。经常引起突变的小鼠 必须繁殖到其他背景菌株以进行最佳研究，这需要通过识别一个或多个目标遗传元素来筛选每一代动物的单个动物。查尔斯·埃尔森（Charles Elson）博士将担任董事，凯西·韦弗（Casey Weaver）博士将担任联合导演。小鼠将在新的Vivarium的障碍条件下安置，该新的Vivarium与新的Shelby研究大楼物理相关。比较医学系的山姆·加特纳（Sam Gartner）博士将担任由DRS的每个项目组成的监督委员会的顾问。 Schoeb和Gartner将监督使用和功能。