CORE--ANIMAL

核心--动物

• 批准号: 7141825
• 负责人: SERGIO A. LIRA
• 金额: $ 27.24万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7141825
• 关键词: animal breeding; animal colony; colitis; disease /disorder model; genetically modified animals; genotype; laboratory mouse; model design /development

This core will provide support for all four projects by performing the animal experiments proposed by each project that utilize different models of experimental colitis. The core's main functions will be: 1) to generate transgenic and knockout and transgenic animals in collaboration with the Mouse Genetics Facility. 2) to genotype and expand lines from transgenic founders and knockouts. 3) to backcross and intercross multiple lines of transgenic and knockout mice. 4) to assist with the experimental colitis models. The Animal Core will take advantage of existing facilities in the Center for Comparative Medicine and Surgery (CCMS) at the Mount Sinai School of Medicine. This is a centralized, shared resource facility that provides veterinary, animal maintenance, and research support to investigators engaged in a variety of research utilizing animal studies. The program is fully accredited by the American Association of Laboratory Animal Care, International. All of the proposed animal experiments will take place in the state-of-the-art barrier facility located on the SC level of the East Building. This is a rodents only facility that offers individually ventilated cages and a fully automated watering system. The Core will provide the technical expertise and support, as well as the necessary equipment and reagents needed for performing all study-related manipulations in mice.

该核心将通过执行动物实验来为所有四个项目提供支持 每个项目都提出了使用不同模型的实验性结肠炎。 核心的主要功能将是： 1）与 小鼠遗传学设施。 2）从转基因创始人和基因淘汰的基因型和扩展线条。 3）进行回杂交和跨多个转基因和基因敲除小鼠。 4）协助实验性结肠炎模型。 动物核心将利用比较中心的现有设施 西奈山医学院的医学与手术（CCMS）。这是一个集中式的 提供兽医，动物维护和研究支持的共享资源设施 使用动物研究从事各种研究的研究人员。该程序是 美国实验室动物护理协会（International）全面认可。所有人 拟议的动物实验将在最先进的障碍设施中进行 位于东楼的SC层。这是只有啮齿动物的设施 单独通风的笼子和全自动浇水系统。核心将提供 技术专长和支持以及必要的设备和试剂 在小鼠中进行所有与研究相关的操作所需的所需。