Animal Model Core

动物模型核心

• 批准号: 7992808
• 负责人: CHARLES O ELSON
• 金额: $ 45.63万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7992808
• 关键词: Address; American; Animal Model; Code; Colitis; Ensure; Flagellin; Goals; Housing; Immune response; Inflammatory Bowel Diseases; Instruction; Knockout Mice; Medicine; Modeling; Mouse Strains; Mus; Pathologic; Pathologist; Quality Control; Reporter; Research Personnel; Specific Pathogen Frees; Surveys; T-Cell Receptor; Tissue Harvesting; Tissues; Transgenic Mice; Transgenic Organisms; adaptive immunity; comparative; cost; germ free condition; insight; mouse model; novel; pathogen; programs; research study

PROJECT SUMMARY (See instructions): Animal Model Core B will provide all mice used in Projects 1, 2, and 3. The Core will be responsible for ordering mice, performing various manipulations required to induce experimental colitis, maintaining a specific pathogen-free barrier facility, performing roufine surveys, to ensure absence of pathogens in the colony, and assist in harvesting tissues from mice. Centralization of these functions in an Animal Model Core provides uniformity and quality control of mice being studied in the various projects, reduces cost, and will help conserve numbers of mice required by ensuring their optimal use in the projects. A second aim of the Animal Model Core is that of centralized pathologic analyses which will be performed by Dr. Trenton Schoeb, an expert veterinary pathologist. Tissue sections from the various projects are coded prior to interpretation and quantitative scoring where needed. This facilitates interactions among the projects and also the comparison of results between them. A third purpose of the Animal Model Core is to provide genetically modified transgenic and knockout mouse strains for use in experiments in the various projects. All of the above Aims have been accomplished in the previous cycle. In regard to the third Aim, during the previous cycle a number of novel genetically-modified mouse lines have been generated in Projects 1, 2, and 3 that have been extremely valuable in addressing innate and adaptive immunity to the microbiota, which is the central goal of this Program Project. These mouse lines include a CBirl flagellin T cell receptor transgenic mouse, an IL-10/Thy1.1 reporter mouse (lOBiT), an IL-17F/Thy1.1 reporter mouse, and an IFNy/Thyl.l reporter mouse. These mouse lines will be housed in Core B and made available to the different projects in this renewal application. Dr. Charies Elson will serve as Director and Dr. Casey Weaver will serve as Co- Director in this Core. Dr. Sam Cartner from the Department of Comparative Medicine will serve as Consultant. An Oversight Committee consisting of the PI of each Project plus Drs. Schoeb and Cartner will oversee the usage and function of the Core.

项目摘要（参见说明）： 动物模型核心 B 将提供项目 1、2 和 3 中使用的所有小鼠。该核心将负责 对小鼠进行排序，进行诱导实验性结肠炎所需的各种操作，维持 特定的无病原体屏障设施，进行常规调查，以确保环境中不存在病原体 菌落，并协助从小鼠身上采集组织。这些功能集中在动物模型核心中 提供各个项目中正在研究的小鼠的一致性和质量控制，降低成本，并将 通过确保项目中的最佳使用来帮助节省所需的小鼠数量。该组织的第二个目标是 动物模型核心是由 Trenton Schoeb 博士进行的集中病理分析， 兽医病理学家专家。来自各个项目的组织切片在解释之前进行编码 并在需要时进行定量评分。这促进了项目之间的互动，也促进了 他们之间的结果比较。动物模型核心的第三个目的是提供基因 改良的转基因和基因敲除小鼠品系用于各种项目的实验。所有的 上述目标已在上一周期完成。关于第三个目标，在上一届会议期间 项目 1、2 和 3 中产生了许多新型转基因小鼠品系， 在解决微生物群的先天性和适应性免疫方面非常有价值，这是 该计划项目的中心目标。这些小鼠品系包括 CBirl 鞭毛蛋白 T 细胞受体转基因 小鼠、IL-10/Thy1.1报告小鼠(1OBiT)、IL-17F/Thy1.1报告小鼠和IFNy/Thyl.l 记者老鼠。这些小鼠品系将被安置在核心 B 中，并可供不同项目使用 此续订申请。 Charies Elson 博士将担任主任，Casey Weaver 博士将担任联合主任 该核心主任。比较医学系的 Sam Cartner 博士将担任 顾问。监督委员会由每个项目的 PI 和 Drs 组成。斯科布和卡特纳将 监督核心的使用和功能。