Real-Time Stereotactic Mass Spectrometry Tissue Analysis for Intraoperative Neuro

术中神经的实时立体定向质谱组织分析

• 批准号: 7981836
• 负责人: Nathalie YR Agar
• 金额: $ 267.59万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-30 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7981836
• 关键词: Accounting; Brain Neoplasms; Clinical; Day Surgery; Detection; Environment; Epilepsy; Excision; Glioblastoma; Glioma; Goals; Histopathology; Image; Intervention; Intracranial Neoplasms; Malignant - descriptor; Mass Spectrum Analysis; Measurement; Modality; Molecular; Molecular Analysis; Neurologic; Neuronavigation; Neurosurgeon; Operating Rooms; Operative Surgical Procedures; Patients; Postoperative Period; Radiology Specialty; Residual Tumors; Risk; Specialized Center; Specificity; Time; Tissues; abstracting; brain tissue; improved; in vivo; intraoperative imaging; multimodality; novel; outcome forecast; tool; treatment strategy; tumor

DESCRIPTION (Provided by the applicant) Abstract: Real-Time Stereotactic Mass Spectrometry Tissue Analysis for Intraoperative Neurosurgical Guidance Clinical and Translational Research Abstract Gliomas account for 40% of intracranial tumors, with close to 70% of them being of anaplastic grade or higher. Their most malignant form, glioblastoma multiforme still resists elaborate treatment strategies with a median survival of 12-15 months, and 2 to 5 years for anaplastic gliomas. To this day, surgery remains the most important, and usually first treatment modality for the majority of brain tumors. The benefits of gross total tumor resection include relief of mass effect, decrease in risk for epilepsy, increase of time to progression, and increase survival time. It is now well accepted that a smaller volume of postoperative residual tumor is associated with an improved prognosis for the patient. The principal challenge and objective of neurosurgical intervention is therefore to maximize the resection of tumor, while minimizing the potential for neurological deficit by preserving critical tissue. Neurosurgeons must then have the capability during surgery to identify diseased tissue as well as critical brain tissue with the highest level of certitude. This project aims to integrate a combined mass spectrometry and neuronavigation platform into the clinical environment for in vivo measurement of tumor boundaries. Mass spectrometry derived signatures will be validated against standard histopathology and correlated to pre- and/or intra- operative radiology imaging. The molecular information could eventually be used to guide clinical decisions in the operating room. A further goal is to create portable tools that may be used in centers without the level of intraoperative imaging available in highly specialized centers. Combination of a novel mass spectrometry approach with a surgical probe will allow simultaneous surgical removal of tissue and in vivo molecular analysis. The proposed project will focus on glioma margin identification in real-time and integrate with a multimodality imaging platform to increase specificity in tumor boundaries detection, for the practice of personalized neurosurgical treatment. Public Health Relevance: In neurosurgical interventions for brain cancers, the principal challenge and objective is to maximize the resection of tumor, while minimizing the potential for neurological deficit by preserving critical tissue. This project aims to develop a real-time molecular analysis of the tissue at stake using mass spectrometry in combination with radiology imaging to guide neurosurgery. The platform being developed for such a complex system can be further adapted to other surgical interventions t in need of highly specific guidance.

描述（申请人提供） 摘要：实时立体定向质谱组织分析用于术中神经外科指导的临床和转化研究摘要胶质瘤占颅内肿瘤的40％，其中近70％的层状成级或更高。它们最恶性的形式多形胶质细胞瘤仍然抵抗精致的治疗策略，中位生存期为12-15个月，而对变性神经胶质瘤的生存期为2至5年。直到今天，手术仍然是大多数脑肿瘤的最重要，通常是第一种治疗方式。总肿瘤切除术的益处包括减轻质量效应，癫痫的风险降低，进展时间增加以及增加生存时间。现在，人们可以接受较少的术后残留肿瘤与患者的预后改善有关。因此，神经外科干预的主要挑战和目标是最大化肿瘤的切除，同时通过保留关键组织来最大程度地减少神经缺陷的潜力。然后，神经外科医生必须在手术期间具有鉴定患病组织以及具有最高水平的关键脑组织的能力。该项目旨在将质谱和神经驱动平台组合到临床环境中，以进行体内测量肿瘤边界。质谱衍生的特征将通过标准的组织病理学进行验证，并与前和/或手术放射学成像相关。该分子信息最终可用于指导手术室中的临床决策。另一个目标是创建便携式工具，这些工具可以用于中心，而没有高度专业中心可用的术中成像水平。新型质谱法与手术探针的结合将允许同时进行组织和体内分子分析的手术去除。拟议的项目将重点介绍实时的神经胶质瘤边缘识别，并与多模式成像平台集成，以增加肿瘤边界检测的特异性，以实践个性化的神经外科治疗。 公共卫生相关性：在脑癌的神经外科干预中，主要挑战和客观是最大程度地切除肿瘤，同时通过保留关键组织来最大程度地减少神经缺陷的潜力。该项目旨在使用质谱与放射学成像结合进行神经外科手术的质谱法对组织的实时分子分析。为这种复杂系统开发的平台可以进一步适应其他手术干预措施，需要高度具体的指导。

项目成果

Molecular imaging of drug transit through the blood-brain barrier with MALDI mass spectrometry imaging.

• DOI: 10.1038/srep02859
• 发表时间: 2013-10-04
• 期刊: SCIENTIFIC REPORTS
• 影响因子: 4.6
• 作者: Liu, Xiaohui;Ide, Jennifer L.;Norton, Isaiah;Marchionni, Mark A.;Ebling, Maritza C.;Wang, Lan Y.;Davis, Erin;Sauvageot, Claire M.;Kesari, Santosh;Kellersberger, Katherine A.;Easterling, Michael L.;Santagata, Sandro;Stuart, Darrin D.;Alberta, John;Agar, Jeffrey N.;Stiles, Charles D.;Agar, Nathalie Y. R.
• 通讯作者: Agar, Nathalie Y. R.

Profiling of adrenocorticotropic hormone and arginine vasopressin in human pituitary gland and tumor thin tissue sections using droplet-based liquid-microjunction surface-sampling-HPLC-ESI-MS-MS.

• DOI: 10.1007/s00216-015-8803-2
• 发表时间: 2015-08
• 期刊: Analytical and bioanalytical chemistry
• 影响因子: 4.3
• 作者: Kertesz V;Calligaris D;Feldman DR;Changelian A;Laws ER;Santagata S;Agar NY;Van Berkel GJ
• 通讯作者: Van Berkel GJ