Primate Core

灵长类核心

• 批准号: 6850623
• 负责人: MAR M SANCHEZ
• 金额: $ 25.35万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6850623
• 关键词: Macaca mulatta; animal care; animal developmental psychology; behavioral /social science research tag; biomedical facility; coping; disease /disorder model; early experience; hypothalamic pituitary adrenal axis; mental disorders; mother child interaction

The Primate Animal Model Core (Core 9002) of the Emory University Center for Neuroscience of Mental Disorders (CNMD) will serve as the source of all non-human primates used by the Research Projects. The primary goal of this Core is to produce, maintain and characterize a non-human primate epigenetic early life stress (ELS) model involving neonatal exposure of rhesus monkey (M. mulatta) mother-infant dyads to social instability in the form of intermittent maternal separations. In this protocol, beginning at 3 months postpartum mothers are removed from social groups composed of 4-5 females and a single male for short (0.5, 3 or 6 hr) intervals. Both the timing and the duration of each separation are determined by a pseudo-randomized schedule. Animals experience a total of 36 separations over a 90 day period (from 3-6 months of age). Over the past 4 years, this Core has produced and characterized approximately 70 animals (approx. 20 animals per year beginning in year 02). Extensive neuroendocrine and behavioral characterization along with structural MRI is performed to determine and verify the phenotype of these animals. One important goal of this Core is to characterize developmental changes in these animals as they pass through significant transitions such as puberty. Animals are assigned to individual Conte Projects as required for specific studies of hypothalamic and extra-hypothalamic CRF systems (Project 0001), immune system function (Project 0009), acoustic startle (Project 0006), cognition and drug abuse liability (Project 0010), multimodal imaging of structural and functional neurocircuits (Project 0013). This procedure offers important advantages and sources of validity: (1) the contingencies imposed by the paradigm are naturalistic, infant development is influenced by unpredictable changes in mother's availability, (2) the demands are imposed during a critical period of development when infants solicit reassurance as they begin to explore their environment, (3) as found in some human psychopathologies, deficits are reflected in the capacity of monkeys to respond to stressful experiences as adults. Comparison of this primate model with the genetic and ELS rodent models (see Core 9001) will permit individual Research Projects to test the thesis that, in diverse species, similar neurocircuits and intracellular mechanisms are impacted by genetic and epigenetic factors across which give rise to a depressive-like syndrome. In addition to production, characterization and maintenance of these animals, Core 9003 will assist in sampling or surgical procedures, drug treatments, or necropsy as required by individual Research Projects. This organization allows consistency in animal handling and implementation of all protocols so that each Conte Research Project receives identically characterized and treated animals for study.

埃默里大学精神障碍神经科学中心 (CNMD) 的灵长类动物模型核心 (Core 9002) 将作为研究项目使用的所有非人类灵长类动物的来源。该核心的主要目标是产生、维持和表征非人类灵长类表观遗传早期生命应激 (ELS) 模型，该模型涉及恒河猴 (M. mulatta) 母婴二人组的新生儿暴露于间歇性母婴形式的社会不稳定。分离。在该方案中，从产后 3 个月开始，母亲会在短时间内（0.5、3 或 6 小时）从由 4-5 名女性和一名男性组成的社会群体中被移除。每次分离的时间和持续时间均由伪随机时间表确定。动物在 90 天内（3-6 个月大）总共经历 36 次分离。在过去 4 年里，该核心已生产并鉴定了约 70 只动物（从 02 年开始每年约 20 只动物）。进行广泛的神经内分泌和行为表征以及结构 MRI 以确定和验证这些动物的表型。该核心的一个重要目标是描述这些动物在经历青春期等重大转变时的发育变化。根据下丘脑和下丘脑外 CRF 系统特定研究的需要，将动物分配到各个 Conte 项目（项目 0001）， 免疫系统功能（项目 0009）、声惊吓（项目 0006）、认知和药物滥用倾向（项目 0010）、结构和功能神经回路的多模态成像（项目 0013）。这个程序提供了重要的优点和有效性来源：（1）范式强加的意外事件是自然的，婴儿的发展受到母亲可用性的不可预测的变化的影响，（2）在婴儿寻求保证的发展关键时期强加要求当猴子开始探索其环境时，(3)正如在一些人类精神病理学中所发现的那样，猴子成年后应对压力经历的能力存在缺陷。将该灵长类动物模型与遗传和 ELS 啮齿动物模型（参见核心 9001）进行比较，将允许各个研究项目测试这样的论点：在不同物种中，相似的神经回路和细胞内机制受到遗传和表观遗传因素的影响，从而产生了抑郁样综合症。除了这些的生产、表征和维护之外 对于动物，Core 9003 将根据个别研究项目的要求协助取样或外科手术、药物治疗或尸检。该组织允许动物处理和所有方案实施的一致性，以便每个 Conte 研究项目都能接收具有相同特征和处理方式的动物进行研究。