Synaptic Integration and Plasticity in Lateral Amygdala

外侧杏仁核的突触整合和可塑性

• 批准号: 6932038
• 负责人: SVETLANA ROSIS
• 金额: $ 2.9万
• 依托单位: NEW YORK UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-30 至 2006-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6932038
• 关键词: action potentials; amygdala; association learning; auditory cortex; auditory stimulus; calcium flux; conditioning; electrostimulus; evoked potentials; fear; juvenile animal; laboratory rat; long term potentiation; neural information processing; neural plasticity; predoctoral investigator; thalamus; tissue /cell culture

DESCRIPTION (provided by applicant): Considerable evidence implicates the lateral nucleus of the amygdala (LA) as a key structure in the acquisition and retention of fear mediated behavior, including auditory fear conditioning in which a neutral conditioned stimulus (CS) such as a tone is paired with an aversive unconditioned stimulus (US) such as a shock. At the cellular level, this type of associative learning is thought to require co-activation of LA neurons by two sets of synaptic inputs; specifically, a weak CS and a strong US. During this synaptic activation, the two inputs are integrated to produce long-term plasticity in LA. In the laboratory, this type of associative plasticity is modeled in vitro using associative long-term potentiation (LTP). The aim of this proposal is to examine how two sets of synaptic inputs might be integrated within the dendritic arbors of a single LA neuron and to show how such integrations might produce long-term plasticity (LTP) in LA, in vitro. Aim I of the proposed studies will address how two sets of synaptic inputs summate in individual LA neurons. Aim II will address how the relative timing of the two inputs affects summation. Aim III will address how the integrative properties of LA neurons can affect the mechanism of LTP induction in LA. Two specific mechanisms, the back-propagating action potential and dendritic regenerative events, proposed to underlie LTP induction in hippocampus will be tested in LA.

描述（由申请人提供）：可观的证据表明，杏仁核（LA）的外侧核是恐惧介导的行为的获取和保留的关键结构，包括听觉恐惧条件，其中中性条件刺激（CS）（例如语调）（例如语调）与厌恶的无条件刺激（我们的抗震动）配对。在细胞水平上，认为这种类型的关联学习需要通过两组突触输入来共同激活LA神经元。具体来说，一个弱的CS和一个强大的我们。在这种突触激活过程中，两个输入被整合以在LA中产生长期可塑性。在实验室中，这种类型的关联可塑性是使用联想长期增强（LTP）在体外建模的。该提案的目的是检查如何在单个LA神经元的树突状街区中整合两组突触输入，并在体外表明该整合可能在LA中产生长期可塑性（LTP）。拟议的研究的目的I将解决两组突触输入如何总结单个LA神经元中的。 AIM II将解决两个输入的相对时机如何影响求和。 AIM III将解决LA神经元的整合性能如何影响LA中LTP诱导的机理。在LA中测试了两种具体机制，即后传播作用潜力和树突再生事件，该机制将在LA中进行测试。