The Genetic Architecture of Aggressive Behavior

攻击行为的遗传结构

• 批准号: 6935104
• 负责人: ALEXIS C EDWARDS
• 金额: $ 3.01万
• 依托单位: NORTH CAROLINA STATE UNIVERSITY RALEIGH
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6935104
• 关键词: Drosophilidae; aggression; behavioral /social science research tag; behavioral genetics; ethology; gene environment interaction; gene expression; gene expression profiling; gene interaction; gene mutation; genetic manipulation; genetic mapping; genetic markers; male; predoctoral investigator; quantitative trait loci

DESCRIPTION (provided by applicant): Aggressive behavior is a quantitative trait, affected by multiple interacting genes with individually small effects, whose expression is sensitive to the environment. I propose several complementary approaches to investigate the genetic basis of aggressive behavior in Drosophila males, using an assay that I developed to rapidly and reproducibly quantify aggressive behavior. Specifically, I will: (1) Map quantitative trait loci (QTLs) affecting variation in aggression. I have localized one QTL with a large effect and will use complementation tests to deficiencies and mutations of positional candidate genes to identify candidate gene(s) corresponding to the QTL. (2) Use artificial selection to derive lines with increased and decreased levels of aggression. This experiment is currently at generation 7.1 will assess whether other traits have evolved as correlated responses to selection for aggression, and use whole genome expression profiling to identify candidate genes whose expression differs significantly between the selection lines. (3) Identify new candidate genes regulating aggressive behavior by quantifying the aggressive behavior of a collection of single P-element insertion lines in a homozygous genetic background.

描述（由申请人提供）：侵略性行为是一种定量性状，受多种相互作用基因的影响，其表达对环境敏感。我提出了几种互补方法，以研究果蝇雄性侵略行为的遗传基础，并使用我开发以快速和可重复地量化攻击性行为的测定法。具体而言，我将：（1）映射量化攻击变化的定量性状基因座（QTL）。我将一个QTL定位为具有较大效果的QTL，并将对位置候选基因的缺陷和突变进行补充测试，以鉴定与QTL相对应的候选基因。 （2）使用人工选择来得出攻击水平升高和降低的线条。该实验目前是在第7.1代的，将评估其他性状是否已演变为对攻击选择的相关反应，并使用整个基因组表达分析来识别候选基因的表达在选择线之间显着差异。 （3）通过量化纯合遗传背景中单个P元素插入线的攻击行为来确定调节侵略行为的新候选基因。