Instrumentation for Genetic and Chemical Screening

遗传和化学筛选仪器

• 批准号: 6733853
• 负责人: TOBIAS MEYER
• 金额: $ 48.84万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6733853
• 关键词: analytical chemistry; biomedical equipment purchase; high throughput technology; robotics

DESCRIPTION (provided by applicant): The objective of this proposal is to create a new high-throughput screening (HTS) facility at Stanford University. Recent advances in genetics and chemical synthesis have fostered novel strategies for biomedical research, and high-throughput liquid-handling and data acquisition instrumentation will enable Stanford faculty and students to exploit these new scientific approaches. For example, the sequencing of entire genomes and the development of techniques to selectively alter gene expression now permit the rapid functional evaluation of essentially any gene product. The screening of natural products and synthetic chemicals for biologically active compounds is also emerging as a powerful complementary approach for deciphering biological processes. The proposed Biomek FX robotic liquid-handling workstation and EnVision multi-label microplate reader will constitute the primary instrumentation systems of this facility, and with this equipment, Stanford researchers will be able to conduct systematic and comprehensive screens of biological systems involving genetic and/or chemical perturbations. Possible approaches include the use of: (1) cDNA libraries for in vivo or in vitro protein expression; (2) siRNA libraries for targeted 'knock-downs' of protein expression; and (3) chemical libraries for the identification of small molecule modulators of specific biological processes. The proposed HTS facility will be developed and managed by the Department of Molecular Pharmacology, and the research projects specifically outlined in this proposal involve studies by its faculty members that pertain to cardiac hypertrophy, neuronal function, drug delivery strategies, embryonic signaling pathways, and mechanisms of oncogenesis. It should be emphasized, however, that these investigations are merely representative of the diverse research activities that the HTS facility will support. Available to the entire Stanford research community and its neighboring institutions, the proposed instrumentation will broadly advance our molecular and cellular understanding of human health and disease by promoting the use of genome-wide approaches in the biomedical sciences and the advancement of chemical biology research.

描述（由申请人提供）：该提案的目的是在斯坦福大学创建新的高通量筛查（HTS）设施。遗传学和化学合成的最新进展促进了生物医学研究的新策略，高通量液体处理和数据获取仪器将使斯坦福大学的教职员工和学生能够利用这些新的科学方法。例如，整个基因组的测序以及选择性改变基因表达的技术的发展现在可以快速对任何基因产物的快速功能评估。对生物活性化合物的天然产物和合成化学物质的筛选也是一种强大的补充生物过程方法。 拟议的Biomek FX机器人液体处理工作站和设想多标签的缩微校准读取器将构成该设施的主要仪器系统，而使用该设备，斯坦福大学的研究人员将能够在涉及遗传和/或化学扰动的生物系统系统的系统和全面筛选中进行系统和全面的筛选。可能的方法包括：（1）cDNA文库用于体内或体外蛋白表达； （2）针对蛋白质表达的目标“敲击”的siRNA文库； （3）用于鉴定特定生物过程的小分子调节剂的化学文库。拟议的HTS设施将由分子药理学系开发和管理，该提案中专门概述的研究项目涉及其教职员工的研究，该研究与心脏肥大，神经元功能，药物输送策略，胚胎信号途径以及Omencenessess的机制有关。但是，应该强调的是，这些调查仅代表HTS设施将支持的各种研究活动的代表。拟议的仪器可用于整个斯坦福大学研究界及其邻近机构，将通过促进生物医学科学中的全基因组方法以及化学生物学研究的发展，广泛地推进我们对人类健康和疾病的分子理解。