Purchase of a Beckman XL-1 Analytical Ultracentrifuge

购买 Beckman XL-1 分析超速离心机

• 批准号: 6440908
• 负责人: DOROTHY BECKETT
• 金额: $ 21.5万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440908
• 关键词: analytical ultracentrifugation; bioenergetics; biomedical equipment purchase; biomimetics; molecular assembly /self assembly; protein structure; stoichiometry

DESCRIPTION (provided by applicant): Nine investigators from three campuses of the University of Maryland seek funding for a Beckman Coulter Optima XL-I analytical ultracentrifuge. The user's group consists of Drs. Beckett (P1), Davis, Isaacs, Julin, Kahn, Munoz, and Greer from the Departments of Chemistry & Biochemistry and Chemical Engineering and the Center for Biological Structure & Organization at UMCP, Dr. David Weber from the Department of Biochemistry and Molecular Biology at the University of Maryland, Baltimore and Dr. Michael Summers from the HHMI and Department of Chemistry & Biochemistry, University of Maryland, Baltimore County. The centrifuge will be used for research in three general areas including analysis of macromolecular assembly in systems of biological interest, determination of the assembly properties of peptides and proteins for folding and high resolution structural studies, and characterization of the energetics and stoichiometries of synthetic biomimetic assemblies. Specific projects include determination of the energetics and stoichiometries of protein-protein interactions in transcriptional regulation and DNA recombination and repair, analysis of model ion channels, and determination of the assembly properties of model peptides for folding studies. The versatility provided by the absorption and interference optics of the Beckman Optima XL-I analytical ultracentrifuge will be essential to the success of the broad range of applications described in this proposal.

描述（由申请人提供）：来自三个校区的九名调查员 马里兰大学为 Beckman Coulter Optima XL-I 寻求资金 分析超速离心机。用户组由 Drs 组成。贝克特（P1）， 来自化学系的 Davis、Isaacs、Julian、Kahn、Munoz 和 Greer & 生物化学与化学工程与生物结构中心 UMCP 的组织，生物化学系的 David Weber 博士和 马里兰大学巴尔的摩分校分子生物学和迈克尔博士 萨默斯 (HHMI) 和美国大学化学与生物化学系 马里兰州巴尔的摩县。该离心机将用于三个方面的研究 一般领域包括系统中大分子组装的分析 生物学意义、肽组装特性的测定和 用于折叠和高分辨率结构研究的蛋白质，以及 合成仿生材料的能量学和化学计量表征 组件。具体项目包括能量学和 转录调控中蛋白质-蛋白质相互作用的化学计量 DNA 重组和修复，模型离子通道分析，以及 确定用于折叠研究的模型肽的组装特性。 吸收和干涉光学器件提供的多功能性 Beckman Optima XL-I 分析超速离心机对于成功至关重要 本提案中描述的广泛应用。

项目成果

Metal-linked dimerization in the iron-dependent regulator from Mycobacterium tuberculosis.

结核分枝杆菌铁依赖性调节因子中的金属连接二聚化。

• 发表时间: 2006-10-17
• 影响因子: 2.9
• 作者: Semavina, Mariya;Beckett, Dorothy;Logan, Timothy M
• 通讯作者: Logan, Timothy M

Bacterial flagellar capping proteins adopt diverse oligomeric states.

细菌鞭毛加帽蛋白采用不同的寡聚状态。

• 发表时间: 2016-09-24
• 影响因子: 7.7
• 作者: Postel, Sandra;Deredge, Daniel;Bonsor, Daniel A;Yu, Xiong;Diederichs, Kay;Helmsing, Saskia;Vromen, Aviv;Friedler, Assaf;Hust, Michael;Egelman, Edward H;Beckett, Dorothy;Wintrode, Patrick L;Sundberg, Eric J
• 通讯作者: Sundberg, Eric J

Diverse oligomeric states of CEACAM IgV domains.

CEACAM IgV 结构域的多种寡聚状态。

• 发表时间: 2015-11-03
• 影响因子: 11.1
• 作者: Bonsor, Daniel A;Günther, Sebastian;Beadenkopf, Robert;Beckett, Dorothy;Sundberg, Eric J
• 通讯作者: Sundberg, Eric J