Cholinergic Regulation of Sleep and Circadian Rhythms

睡眠和昼夜节律的胆碱能调节

• 批准号: 7117615
• 负责人: Sabra M Abbott
• 金额: $ 7.67万
• 依托单位: UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-09 至 2008-08-08
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7117615
• 关键词: REM sleep; acetylcholine; anticholinergic agent; atropine; behavior test; biological clocks; brain stem; carbachol; cholinergic agents; circadian rhythms; electrostimulus; ethology; laboratory rat; male; neurochemistry; neuropharmacology; neurophysiology; neuroregulation; predoctoral investigator; sleep; sleep deprivation; suprachiasmatic nucleus

DESCRIPTION (provided by applicant): The central pacemaker for mammalian circadian rhythms, the suprachiasmatic nucleus (SCN), receives a wide variety of neurochemical inputs, including cholinergic projections originating in the brainstem and the basal forebrain. The circadian pattern of cholinergic influence and the role of this signal in the functioning of the SCN are still being determined. Early data suggested that acetylcholine caused phase delays in wheel running behavior when applied early in the night, and phase advances when applied late in the night, similar to the response seen following light exposure. However in vitro evidence in both rats and mice indicates that when cholinergic agonists are applied directly to the SCN, either in vivo or in vitro, they cause phase advances throughout the night, with a maximal response at circadian time (CT) 18 in rats and CT 20 in mice. This proposal will attempt to further characterize the cholinergic influence on the SCN, and test the hypothesis that this cholinergic input plays a role in regulating the sleep-wake and circadian cycles of the animal.

描述（由申请人提供）： 哺乳动物昼夜节律的中央起搏器视交叉上核（SCN）接收多种神经化学输入，包括源自脑干和基底前脑的胆碱能投射。胆碱能影响的昼夜节律模式以及该信号在 SCN 功能中的作用仍在确定中。早期数据表明，在深夜使用乙酰胆碱时，乙酰胆碱会导致车轮运行行为的相位延迟，而在深夜使用时会导致相位提前，类似于光照后的反应。然而，大鼠和小鼠的体外证据表明，当胆碱能激动剂直接应用于 SCN 时，无论是体内还是体外，它们都会导致整个晚上的相位提前，在大鼠和小鼠的昼夜节律时间 (CT) 18 时反应最大。小鼠 CT 20。该提案将尝试进一步表征胆碱能对 SCN 的影响，并检验胆碱能输入在调节动物的睡眠-觉醒和昼夜节律周期中发挥作用的假设。