Structural studies of phenyl propanoid metabolism

苯丙素代谢的结构研究

• 批准号: 6918038
• 负责人: CHULHEE KANG
• 金额: $ 21.63万
• 依托单位: WASHINGTON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6918038
• 关键词: X ray crystallography; active sites; alcohol dehydrogenase; binding proteins; branched chain aminoacid; computer program /software; crystallization; enzyme activity; enzyme biosynthesis; enzyme mechanism; enzyme structure; enzyme substrate analog; enzyme substrate complex; flavonoids; intermolecular interaction; lignans; oxidoreductase; protein folding; protein sequence; protein structure function; stereochemistry

DESCRIPTION (provided by applicant): The long-term objectives of this research are to establish the detailed nature of the biochemical processes involved in phenylpropanoid metabolism, which together constitutes approximately 30% of the carbon in vascular plants. This includes the enzymes and protein factors involved in biochemical pathways to the medicinally important lignans and isoflavonoids, as well as to the structural plant polymeric lignins. Of these the lignans and isoflavonoids have important roles in human health protection (e.g., chemoprotection against various cancers, in lowering blood cholesterol levels) in treatment of various cancers (e.g., testicular), and in prevention of certain allergies.This particular proposal aims to define the mode of action of the first known protein able to control the binding and orientation of plant phenols (monolignol derived) in order that stereoselective coupling can occur. It is considered that definition of the functional motifs on this protein will aid greatly in identification of other analogous proteins of similar function. Additionally, we wish to examine three aromatic alcohol dehydrogenases involved in key steps to these medicinally important lignans and isoflavonoids, in order to determine how differing enantiospecificity is achieved albeit in each case all going through similar enone intermediates. The primary emphasis of the study will thus employ x-ray crystallographic analysis of each protein with substrates, substrate analogs and inhibitors.

描述（由申请人提供）：本研究的长期目标是确定苯丙素代谢所涉及的生化过程的详细性质，苯丙素代谢总共构成了维管植物中约 30% 的碳。这包括参与重要药用木脂素和异黄酮类化合物以及结构植物聚合木质素的生化途径的酶和蛋白质因子。其中，木脂素和异黄酮类化合物在人类健康保护（例如，针对各种癌症的化学保护、降低血液胆固醇水平）、治疗各种癌症（例如睾丸）以及预防某些过敏方面具有重要作用。该特别提案旨在定义了第一个已知蛋白质的作用模式，该蛋白质能够控制植物酚（monolignol 衍生）的结合和方向，以便可以发生立体选择性偶联。人们认为，对该蛋白质的功能基序的定义将极大地有助于鉴定具有相似功能的其他类似蛋白质。此外，我们希望检查参与这些药用重要木脂素和异黄酮类化合物关键步骤的三种芳香醇脱氢酶，以确定如何实现不同的对映特异性，尽管在每种情况下都经过类似的烯酮中间体。因此，该研究的主要重点将是对每种蛋白质及其底物、底物类似物和抑制剂进行 X 射线晶体学分析。

项目成果

The unique hydrogen bonded water in the reduced form of Clostridium pasteurianum rubredoxin and its possible role in electron transfer.

巴氏梭菌红氧还蛋白还原形式中独特的氢键水及其在电子转移中的可能作用。

• DOI: 10.1007/s00775-004-0542-3
• 发表时间: 2004
• 期刊: Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry
• 作者: Park,IlYeong;Youn,Buhyun;Harley,JillL;Eidsness,MarlyK;Smith,Eugene;Ichiye,Toshiko;Kang,ChulHee
• 通讯作者: Kang,ChulHee

Crystallographic studies of V44 mutants of Clostridium pasteurianum rubredoxin: effects of side-chain size on reduction potential.

巴氏梭菌红氧还蛋白 V44 突变体的晶体学研究：侧链大小对还原电位的影响。

• DOI: 10.1002/prot.20243
• 发表时间: 2004
• 期刊: Proteins
• 影响因子: 2.9
• 作者: Park,IlYeong;Eidsness,MarlyK;Lin,I-Jin;Gebel,ErikaB;Youn,Buhyun;Harley,JillL;Machonkin,TimothyE;Frederick,RonnieO;Markley,JohnL;Smith,EugeneT;Ichiye,Toshiko;Kang,Chulhee
• 通讯作者: Kang,Chulhee

Chavicol formation in sweet basil (Ocimum basilicum): cleavage of an esterified C9 hydroxyl group with NAD(P)H-dependent reduction.

甜罗勒 (Ocimum basilicum) 中 Chavicol 的形成：通过 NAD(P)H 依赖性还原裂解酯化的 C9 羟基。

• DOI: 10.1039/b605407b
• 发表时间: 2006
• 期刊: Organic & biomolecular chemistry.
• 作者: Vassao,DanielG;Gang,DavidR;Koeduka,Takao;Jackson,Brenda;Pichersky,Eran;Davin,LaurenceB;Lewis,NormanG
• 通讯作者: Lewis,NormanG