A THALIANA DOUBLE BOND REDUCTASE APO-FORM, BINARY AND TERNARY COMPLEXES

THALIANA 双键还原酶 APO 型、二元和三元复合物

• 批准号: 7721265
• 负责人: CHULHEE KANG
• 金额: $ 2.12万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-05-15 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7721265
• 关键词: Anabolism; Antifungal Agents; Antioxidants; Antiviral Agents; Blood Vessels; Cell Wall; Cinnamoyl-CoA reductase; Cinnamyl-alcohol dehydrogenase; Complex; Computer Retrieval of Information on Scientific Projects Database; Enzymes; Etoposide; Evolution; Funding; Goals; Grant; Ingestion; Institution; Lignans; Lignin; Malignant Neoplasms; Monoterpenes; Object Attachment; Oxidoreductase; Paclitaxel; Plants; Play; Podophyllotoxin; Property; Proteins; Psychological reinforcement; Regulation; Research; Research Personnel; Resolution; Resources; Roentgen Rays; Role; Source; Structure-Activity Relationship; Teniposide; United States National Institutes of Health; Vascular Plant; antimicrobial; base; cancer therapy; enterodiol; enzyme pathway; lariciresinol reductase; leukemia; lung small cell carcinoma; pinoresinol; prevent; secoisolariciresinol

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The evolution of plant forms from an aquatic to a land base was accompanied by formation of a vast plethora of phenolic components in the vascular plants, of which the lignins, lignans and monoterpene are the most abundant. Together they play important roles in plant defense and cell wall reinforcement, such as with the lignans having antimicrobial, antifungal, antiviral and antioxidant properties, whereas the lignins have structural roles in support of vascular apparatus integrity. Some lignans and monoterpene derivatives find widespread application in cancer treatment, e.g., the podophyllotoxin derivatives, teniposide, etoposide, etopohos and taxol which are used in treatment of germinal testicular and small cell lung cancers, and for certain forms of leukemia. Others,such as enterodiol/enterolactone, are believed to have cancer-preventing properties and are formed through dietary ingestion of lignans (and possibly lignin) precursors. The goal of this project is thus to define the structure function relationships of these key proteins and enzymes in lignan, lignin and monolignol biosynthesis through high resolution X-ray structural analyses. We describe and compare what is now known about the mechanistic (regiospecificity and enantiospecificity) basis of the lignan pathway enzymes, pinoresinol-lariciresinol reductase and secoisolariciresinol dehydrogenase as well as of various monolignol pathway enzymes (e.g., cinnamyl alcohol dehydrogenase, arylpropenal double bond reductase, cinnamoyl CoA reductase, etc.) The information gained from these studies will be very useful for exploring the regulation of phenylpropanoid biosynthesis for ecological plant protection, and for the industrial-scale regiospecific and stereospecific synthesis of these pharmacologically active substances.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此，可以在其他清晰的条目中表示。列出的机构是 对于中心，这不一定是调查员的机构。 植物形式从水生的进化到土地基础，伴随着在血管植物中形成大量酚类成分的植物，其中木质素，木质蛋白和单苯乙烯是最丰富的。 它们共同在植物防御和细胞壁增强中起着重要作用，例如具有抗菌，抗真菌，抗病毒药物和抗氧化特性的木糖，而木质素在支持血管仪的完整性方面具有结构作用。一些木烷和单苯乙烯衍生物在癌症治疗中发现了广泛的应用，例如，podophyllotoxin衍生物，teniposide，teniposide，etopohos，etopohos和紫杉醇用于治疗生发性睾丸和小细胞肺肺癌，以及某些形式的白血病。据信，其他其他人，例如肠二醇/肠痛具有预防癌症的特性，并通过饮食中摄入木质（甚至可能是木质素）前体而形成。因此，该项目的目的是通过高分辨率的X射线结构分析来定义木质素，木质素和单极生物合成中这些关键蛋白和酶的结构功能关系。 我们描述并比较了现在有关木质途径酶的机械性（防治性和对晶体特定性）基础的了解和比较的知名度CINNAMOYL COA还原酶等。）这些研究所获得的信息将非常有用，对于探索苯基丙烷生物合成的调节，以进行生态植物保护，以及工业规模的养殖型和立体特定的这些药理活性物质的合成。