Nanoparticle Generator for Animal Inhalation Experiments

用于动物吸入实验的纳米颗粒发生器

• 批准号: 6880363
• 负责人: AMIR A NAQWI
• 金额: $ 10万
• 依托单位: POWERSCOPE, INC
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2006-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6880363
• 关键词: biomedical equipment development; enzyme activity; high performance liquid chromatography; macromolecule; nanotechnology; particle; pulmonary respiration; small molecule

DESCRIPTION (provided by applicant): Objective of this research is to generate airborne fine particles around 100 nm, using high-frequency ultrasonic nebulization and subsequent spray drying. Such a particle generator would be valuable for inhalation experiments on small laboratory animals. Future applications of this technology would include generation of nano-powders of drugs for the purpose of efficient delivery and high bioavailability, as well as fluorescent nanoprobes for cell-level studies of biochemical processes. Development of the proposed device poses several technical challenges. High-energy acoustic waves may cause thermal or mechanical damage to the drug that needs to be controlled using efficient cooling, intermittent operation of the acoustic actuator and the choice of suitable solvents. The proposed development would be accomplished through experimental research, utilizing several advanced instruments as diagnostic tools. Drop sizes prior to spray drying will be measured using a real-time ensemble laser diffraction instrument. After completion of the drying process, solid particles will be measured using an electro-mobility based particle sizer. Evaluation and testing will cover particles of small molecules as well as macromolecules, i.e. proteins, whose potency after nebulization will be tested by measuring their enzymatic activity. HPLC techniques will be used to verify the integrity of small molecule drugs.

描述（由申请人提供）：本研究的目的是利用高频超声波雾化和随后的喷雾干燥产生空气中约 100 nm 的细颗粒。这种粒子发生器对于小型实验动物的吸入实验非常有价值。该技术的未来应用将包括生成药物纳米粉末，以实现高效递送和高生物利用度，以及用于细胞水平生化过程研究的荧光纳米探针。 所提出的设备的开发提出了一些技术挑战。高能声波可能会对药物造成热或机械损伤，需要通过有效冷却、声致动器的间歇操作和选择合适的溶剂来控制。 拟议的开发将通过实验研究来完成，利用多种先进仪器作为诊断工具。喷雾干燥前的液滴尺寸将使用实时集成激光衍射仪进行测量。干燥过程完成后，将使用基于电动势的粒度仪测量固体颗粒。 评估和测试将涵盖小分子颗粒以及大分子（即蛋白质），其雾化后的效力将通过测量其酶活性来测试。 HPLC技术将用于验证小分子药物的完整性。

项目成果

In Vitro Evaluation of a Device for Intra-Pulmonary Aerosol Generation and Delivery.

• DOI: 10.1080/02786826.2015.1067670
• 发表时间: 2015-09-01
• 期刊: Aerosol science and technology : the journal of the American Association for Aerosol Research
• 作者: Syedain ZH;Naqwi AA;Dolovich M;Somani A
• 通讯作者: Somani A

Measurement of the distribution of aerosols among mouse lobes by fluorescent imaging.

通过荧光成像测量小鼠肺叶中气溶胶的分布。

• DOI: 10.1016/j.ab.2010.04.005
• 发表时间: 2010
• 期刊: Analytical biochemistry
• 影响因子: 2.9
• 作者: Yi,Dandan;Price,Andrew;Panoskaltsis-Mortari,Angela;Naqwi,Amir;Wiedmann,TimothyScott
• 通讯作者: Wiedmann,TimothyScott