Protein-Display Beads

蛋白质展示珠

• 批准号: 6992414
• 负责人: John Thomas Mulligan
• 金额: $ 13.32万
• 依托单位: BLUE HERON BIOTECHNOLOGY, INC.
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6992414
• 关键词: DNA; biotechnology; diffusion; gene expression; high throughput technology; nucleic acid sequence; polymerase chain reaction; polymers; protein structure function; technology /technique development

DESCRIPTION (provided by applicant): In this application we propose to develop a simple and economical method for producing protein-display beads using a novel approach to controlling diffusion. Each bead will carry a different DNA sequence and the protein encoded by that sequence, and the method will produce beads carrying hundreds of millions of different sequences in a single reaction. The beads will be used in a new, extremely high-throughput technology for studying protein function. This new approach combines the data-intensive style of genomics and elements of sequencing technology in a way that can be routinely applied to many different proteins. We plan to commercialize the technology by using it to characterize and optimize proteins of medical and industrial value. We will provide the functional characterization of proteins as a new service for our existing customers in the biotechnology and pharmaceutical industries, and use the technology on internal research projects aimed at developing new industrial and therapeutic proteins. The protein-display beads can also be used in a selection for superior proteins from a pool of protein variants, This could itself be the basis for new products for our company.

描述（由申请人提供）：在本申请中，我们建议开发一种简单且经济的方法，使用控制扩散的新方法来生产蛋白质展示珠。每个珠子将携带不同的DNA序列和该序列编码的蛋白质，并且该方法将在一次反应中产生携带数亿个不同序列的珠子。这些珠子将用于一种新的、极高通量的技术来研究蛋白质功能。这种新方法结合了基因组学的数据密集型和测序技术的元素，可以常规应用于许多不同的蛋白质。 我们计划利用该技术来表征和优化具有医疗和工业价值的蛋白质，从而将该技术商业化。我们将为生物技术和制药行业的现有客户提供蛋白质的功能表征作为一项新服务，并将该技术用于旨在开发新的工业和治疗性蛋白质的内部研究项目。蛋白质展示珠还可以用于从蛋白质变体库中选择优质蛋白质，这本身就可以成为我们公司新产品的基础。