Molecular Microarray Core

分子微阵列核心

• 批准号: 7122963
• 负责人: EDWARD A FOX
• 金额: $ 10.15万
• 依托单位: DANA-FARBER CANCER INST
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7122963
• 关键词: acute lymphocytic leukemia; biomedical facility; biotechnology; comparative genomic hybridization; gene expression; gene expression profiling; genetic transcription; human T cell leukemia; microarray technology; neoplastic transformation; nucleic acid amplification techniques; thymus neoplasms

DESCRIPTION (provided by applicant): The role of the Microarray Core will be to carry out genome-wide analyses of transcriptional changes and of gene copy number changes that result from either experimentally induced or clinically arising aberrations in genes that regulate genomic stability, cell survival, cell cycle signaling and developmental maturation in T cell oncogenesis. Gene expression profiling will be carried out on specimens from projects 1, 2 and 4, as a means to discover the transcriptional consequences of perturbing molecules that are critical in these processes. Array Comparative Genomic Hybridization will be performed to detect and quantify gene loss and gene amplification in tumors from experimental animals in project 4. It is anticipated that both types of microarray studies will identify genes whose activity, and in some cases, structure, are disrupted by abnormalities in the key regulatory proteins involved in T cell malignancy. The following services will be provided by the Microarray Core: 1. Starting with RNA provided by investigators, produce transcriptional profiling data using mouse or human oligonucleotide microarrays. 2. Starting with genomic DNA provided by investigators, produce genomewide gene copy number determinations using mouse or human oligonucleotide microarrays.

描述（由申请人提供）：微阵列核心的作用将是对转录变化的全基因组进行分析以及基因拷贝数的变化，这些变化是由实验诱导的或临床上引起的基因的临床差异，这些基因的畸变，这些基因的基因组稳定性，细胞存活，细胞周期信号传导和T细胞过度的发展。基因表达分析将在项目1、2和4的标本上进行，作为发现在这些过程中至关重要的扰动分子的转录后果的一种手段。将进行阵列比较基因组杂交，以检测和量化项目4中实验动物的肿瘤中的基因丧失和基因扩增。预计两种类型的微阵列研究都将鉴定其活性，在某些情况下，在某些情况下，在某些情况下会被关键调节蛋白中涉及T细胞不良的主要调节蛋白中的异常扰动。以下服务将由微阵列核心提供： 1。从研究人员提供的RNA开始，使用小鼠或人类寡核苷酸微阵列产生转录分析数据。 2。从研究人员提供的基因组DNA开始，使用小鼠或人类寡核苷酸微阵列确定全基因组基因拷贝数。