Core--Genomics

核心--基因组学

• 批准号: 6912417
• 负责人: BRIAN WICKES
• 金额: $ 7.31万
• 依托单位: UNIVERSITY OF TEXAS SAN ANTONIO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-08 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6912417
• 关键词: Francisella tularensis; biomedical facility; biotechnology; bioterrorism /chemical warfare; functional /structural genomics; microarray technology; nucleic acid chemical synthesis; nucleic acid quantitation /detection; nucleic acid sequence; oligonucleotides; pathologic process; polymerase chain reaction; virulence

DESCRIPTION (provided by applicant): Francisella tularensis is a highly infectious pathogen that can be acquired by the aerosol route. As such, it has been classified as a Category A select agent by the CDC. No licensed vaccine is available for protection against infection by this organism. Genomic approaches, relying heavily on microarray development and analysis, will be key to unlocking the mysteries concerning the virulence of the organism, as well as the response of the host, during infection. This Core will be focused on support for all of the Projects in this PO1 application with the following aims: Aim 1 is construction and utilization of a F. tularensis Schu4 oligonucleotide microarray; and, Aim 2 is support services to the program project for DNA sequencing, oligonucleotide production, and other nucleic acid detection/analytical functions (e.g., Real Time PCR and Typhoon Imaging system). With the support of this Core, the individual projects will lead to novel discoveries in the fields of Francisella pathogenesis and immunity.

描述（由申请人提供）：土拉弗朗西斯菌是一种高度传染性的病原体，可以通过气溶胶途径获得。因此，它被归类为 A 类 由CDC选择代理。没有获得许可的疫苗可以预防这种感染 生物。基因组方法在很大程度上依赖于微阵列的开发和分析，将成为解开有关生物体毒力以及感染期间宿主反应之谜的关键。该核心将重点支持 PO1 申请中的所有项目，目标如下： 目标 1 是构建和利用 F. tularensis Schu4 寡核苷酸微阵列；目标 2 是为 DNA 测序、寡核苷酸生产和其他核酸检测/分析功能（例如实时 PCR 和 Typhoon 成像系统）项目提供支持服务。在该核心的支持下，各个项目将在弗朗西斯菌发病机制和免疫领域带来新的发现。