Method for Quantitative Fluorescence Sensing in Tissue

组织中定量荧光传感的方法

• 批准号: 6906475
• 负责人: Mary-Ann Mycek
• 金额: $ 17.59万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6906475
• 关键词: articular cartilage; biotechnology; chondrocytes; collagen; endoscopy; fiber optics; fluorescence spectrometry; fluorescent dye /probe; gastrointestinal epithelium; gastrointestinal imaging /visualization; high throughput technology; ionophores; laser spectrometry; method development; noninvasive diagnosis; phantom model; technology /technique development; tissue engineering

DESCRIPTION (provided by applicant): Fluorescence spectroscopy and imaging are being applied to a wide variety of noninvasive biomedical studies for in vitro and in vivo diagnostics. One of the challenges in tissue fluorescence is to obtain quantitative information on the molecular fluorophores in tissues despite the detected signal variability caused by tissue absorption and scattering. The overall goal of this R21 application is to develop, optimize, and validate a technology and method to rapidly, non-invasively, and quantitatively sense endogenous and exogenous fluorophores in tissues. The method combines both experimental and computational approaches, and will employ novel computational codes developed to model time-resolved excitation and fluorescent light propagation in complex tissues approximately 100 times faster than current methods. To illustrate the broad utility of the method, it will be applied to two distinct biomedical problems. A multidisciplinary team of researchers with expertise in biomedical optics, gastrointestinal endoscopy, and tissue engineering has been assembled to ensure that these goals are achieved. The specific aims are: (1) To develop a novel technology and method for rapid, quantitative, non-invasive molecular sensing in homogeneous tissues using time-resolved laser spectroscopy and the principles of tissue optics; (2) To extend the technology developed in Aim 1 to include application to inhomogeneous tissues with multiple layers, for the development and optimization of fiber-optic probes compatible with clinical endoscopy; (3) To extend the technology developed in Aim 1 to include application to tissues with multiple fluorophores per layer, to enable tissue engineers to quantitatively and noninvasively monitor the composition, structure, and function of their constructs. The technologies and methods developed and validated here are broadly applicable to a variety of applications in quantitative tissue fluorescence spectroscopy and imaging. To facilitate the generalized use of this approach, the computational codes can be efficiently disseminated to the scientific community via a download link or interactive interface on the Pl's web-site.

描述（由申请人提供）： 荧光光谱和成像被应用于体外和体内诊断的多种非侵入性生物医学研究。尽管发现组织吸收和散射引起的信号变异性，但组织荧光中的挑战之一是获得组织中分子荧光团的定量信息。该R21应用的总体目标是开发，优化和验证一种技术和方法，以快速，非侵入性和定量感知到组织中的内源性和外源性荧光团。该方法结合了实验方法和计算方法，并将采用开发的新型计算代码，以模拟复杂组织中时间分辨的激发和荧光传播的速度，比当前方法快100倍。 为了说明该方法的广泛效用，它将应用于两个不同的生物医学问题。已经组装了一支具有生物医学光学，胃肠道内窥镜和组织工程专业知识的研究人员的多学科团队，以确保实现这些目标。 具体目的是：（1）开发一种新的技术和方法，用于使用时间分辨的激光光谱和组织光学原理在均质组织中快速，定量，非侵入性分子感测； （2）扩展在AIM 1中开发的技术，以包括多个层的不均匀组织的应用，以开发和优化与临床内窥镜兼容的光纤探针； （3）扩展AIM 1中开发的技术，以将应用于每层多个荧光团的组织应用，以使组织工程师能够定量和非侵入性地监测其构建体的组成，结构和功能。 这里开发和验证的技术和方法广泛适用于定量组织荧光光谱和成像中的各种应用。为了促进这种方法的广泛使用，可以通过PL的网站上的下载链接或交互式界面有效地将计算代码有效地传播到科学界。